Clinical application of genetic tests regarding cariogenicity of Streptococcus mutans

• Project/Area Number: 23792506
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Social dentistry
• Research Institution: Niigata University
• Principal Investigator: KANEKO Noboru 新潟大学, 医歯学系, 助教 (00397126)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 歯学 / Streptococcus mutans / 齲蝕原性 / ミュータンスレンサ球菌

Research Abstract

An important virulence factor of S. mutans is a glucosyltransferase synthesizing water-insoluble glucan (WIG) from sucrose. We performed sequencing of the gtfB gene of S. mutans isolates derived from primary school students.
GtfB coded by gtfB gene possess two functional domain. The carboxyl-terminal portion of GtfB is called the glucan binding domain and is coded by the direct repeating units. The results of this study indicated that the substitution of 33rd amino acid Gly to Ser in the repeating unit is associated with WIG synthesis by S. mutans. And we revealed that the p.G33S substitution is associated with caries development in 1 year in the students using a fluoride mouth rinse.

Research Products

• [Journal Article] Association between glucan synthesis by Streptococcus mutans and caries incidence in schoolchildren receiving a fluoride mouth rinse (2012)
  • Author(s): Umetsu H, Kaneko N, Yoshihara A, Sakuma S, Hanada N, Miyazaki H
  • Journal Title: Oral Health Prev Dent Volume: 10(2) Pages: 161-166
  • Peer Reviewed
• [Journal Article] Serum antibody to Porphyromonas gingivalis in chronic kidney disease (2012)
  • Author(s): Iwasaki M, Taylor GW, Manz MC, Kaneko N, Imai S, Yoshihara A, Miyazaki H
  • Journal Title: J Dent Res Volume: 91(9) Pages: 828-833
  • Peer Reviewed
• [Journal Article] Moistened techniques considered for patients'comfort and operators'ease in dental treatment (2012)
  • Author(s): Ito K, Funayama S, Katsura K, Kaneko N, Nohno K, Saito M, Yamada A, Sumida Y, Inoue M
  • Journal Title: Int J Oral-Medical Sci Volume: 11(2) Pages: 85-89
  • Peer Reviewed
• [Journal Article] Association between glucan synthesis by Streptococcus mutans and caries incidence in schoolchildren receiving a fluoride mouth rinse (2012)
  • Author(s): Umetsu H, Kaneko N, Yoshihara A, Sakuma S, Hanada N, Miyazaki H
  • Journal Title: Oral Health Preventive Dent Volume: 10
  • Peer Reviewed
• [Presentation] Streptococcus mutansのGtfB酵素C末端側におけるアミノ酸配列と齲蝕との関連性 (2014)
  • Author(s): 金子昇,濃野要,葭原明弘,花田信弘,宮﨑秀夫
  • Organizer: 第63回日本口腔衛生学会総会
  • Place of Presentation: 熊本市,日本口腔衛生学会雑誌,64(2), 207, 2014
• [Presentation] Streptococcus mutansのGtfB酵素C末端側におけるアミノ酸配列と齲蝕との関連性 (2014)
  • Author(s): 金子 昇、濃野 要、葭原明弘、花田信弘、宮埼秀夫
  • Organizer: 第63回日本口腔衛生学会・総会
  • Place of Presentation: 熊本市国際交流会館
• [Presentation] Factors associated with vaginal dryness; comparison with multiple regions (2013)
  • Author(s): Ito K, Takamatsu K, Nohno K, Yamada A, Funayama S, Katsura K, Kaneko N, Hatakeyama A, Ogawa M, Inoue M
  • Organizer: The 5th Scientific Meeting of the Asia Pacific Menopause Federation
  • Place of Presentation: Tokyo (Japan)
• [Presentation] 口腔乾燥症診断シート導入の試み (2013)
  • Author(s): 伊藤加代子,船山さおり,勝良剛詞,金子昇,濃野要,山田亜紀,井上誠
  • Organizer: 第23回日本口腔内科学会第26回日本口腔診断学会合同学術大会
  • Place of Presentation: 東京,日本口腔診断学会総会プログラム・抄録集,125, 2013
• [Presentation] ドライマウス診断におけるpulse Doppler ultrasonographyの可能性 (2013)
  • Author(s): 勝良剛詞,伊藤加代子,濃野要,船山さおり,金子昇,山田亜紀,林孝文
  • Organizer: 第23回日本口腔内科学会第26回日本口腔診断学会合同学術大会
  • Place of Presentation: 東京,日本口腔診断学会総会プログラム・抄録集,134, 2013
• [Presentation] 男性におけるドライシンドローム有訴率に関する検討 (2013)
  • Author(s): 伊藤加代子,濃野要,船山さおり,金子昇,山田亜紀,畠山文,佐々木綾子,井上誠
  • Organizer: 日本老年歯科医学会第24回学術大会
  • Place of Presentation: 大阪市,老年歯科医学,28(2): 225, 2013
• [Presentation] Streptococcus mutansのGtfB酵素C末端側におけるアミノ酸配列と非水溶性グルカン合成能 (2012)
  • Author(s): 金子 昇，濃野 要，葭原明弘，花田信弘，宮﨑秀夫
  • Organizer: 第61回日本口腔衛生学会総会
  • Place of Presentation: 横須賀市
• [Presentation] Streptococcus mutansのgtfB遺伝子におけるdirect repeatと非水溶性グルカン合成能 (2012)
  • Author(s): 金子 昇，花田信弘
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎ブリックホール（長崎市）
• [Presentation] Streptococcus mutans臨床分離株のgtfB遺伝子における直列反復配列と非水溶性グルカン合成能 (2011)
  • Author(s): 金子 昇，濃野 要，葭原明弘，花田信弘，宮崎秀夫
  • Organizer: 第60回 日本口腔衛生学会・総会
  • Place of Presentation: 日本大学松戸歯学部（松戸市）
• [Presentation] 乳幼児検診に併設し実施する個別指導による歯科保健行動の変容 (2011)
  • Author(s): 坂入久美子，葭原明弘，金子 昇，林 悠子，藤山友紀，宮崎秀夫
  • Organizer: 第22回甲信越北陸口腔保健研究会総会・学術大会
  • Place of Presentation: 新潟大学歯学部（新潟市）