Structure of oral indigenous microbiota for evaluation of oral health

• Project/Area Number: 23792517
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Social dentistry
• Research Institution: Kyushu University
• Principal Investigator: TAKESHITA Toru 九州大学, 歯学研究院, 助教 (50546471)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2011: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 口腔常在フローラ / 16S rRNA / 次世代シーケンサー / 口腔フローラ / 健康 / 高齢者

Research Abstract

This investigation revealed that salivary bacterial population of the elderly with healthy oral condition exhibited characteristic composition. Neisseria species were characteristically predominant in their microbiota compared with the patients visiting dental offices. Interaction of Neisseria with other commensal bacteria or host might be involved with maintenance of oral health. This result suggests that Neisseria would be a target for a new prevention approach of oral diseases

Research Products

• [Journal Article] Discrimination of the oral microbiota associated with high hydrogen sulfide and methyl mercaptan production (2012)
  • Author(s): Takeshita T, Suzuki N, Nakano Y, Yasui M, Yoneda M, Shimazaki Y, Hirofuji T, Yamashita Y
  • Journal Title: Scientific Reports Volume: 2 Pages: 215-215
  • Peer Reviewed
• [Journal Article] Molecular characterization of fungal populations on the tongue dorsum of institutionalized elderly adults (2012)
  • Author(s): Li H, Takeshita T, Furuta M, Tomioka M, Shibata Y, Makimura K, Yamashita Y
  • Journal Title: Oral Diseases Volume: 18 Pages: 771-777
  • Peer Reviewed
• [Journal Article] Compositional stability of a salivary bacterial population against supragingival microbiota shift following periodontal therapy (2012)
  • Author(s): Yamanaka W, Takeshita T, Shibata Y, Matsuo K, Eshima N, Yokoyama T, Yamashita Y
  • Journal Title: PLoS One Volume: 7
  • Peer Reviewed
• [Journal Article] Discrimination of the oral microbiota associated with high hydrogen sulfide and methyl mercaptan production (2012)
  • Author(s): T.Takeshita
  • Journal Title: Scientific Reports Volume: 2 Issue: 1 Pages: 215-215
  • DOI: 10.1038/srep00215
  • Peer Reviewed
• [Journal Article] Molecular characterization of fungal populations on the tongue dorsum of institutionalized elderly adults. (2012)
  • Author(s): Li H.
  • Journal Title: Oral Diseases Volume: 18 Issue: 8 Pages: 771-777
  • DOI: 10.1111/j.1601-0825.2012.01944.x
  • Peer Reviewed
• [Journal Article] D.D.S. Structural Stability of a Salivary Bacterial Population against Supragingival Microbiota Shift Following Periodontal Thera (2012)
  • Author(s): Wataru Yamanaka, Toru Takeshita, Ph.D, Yukie Shibata; Kazuki Matsuo; Nobuoki Eshima, Takeshi Yokoyama; Yoshihisa Yamashita, Ph.D.
  • Journal Title: PLoS ONE Volume: 7, 8, e42806 Issue: 8 Pages: e42806-e42806
  • DOI: 10.1371/journal.pone.0042806
  • Peer Reviewed
• [Journal Article] Enteral Tube Feeding Alters the Oral Indigenous Microbiota in Elderly Adults (2011)
  • Author(s): Takeshita T, Yasui M, Tomioka M, Yamashita Y
  • Journal Title: Appl Environ Microbiol Volume: 77 Pages: 6739-6745
  • Peer Reviewed
• [Presentation] う蝕経験のない成人の口腔常在フローラの細菌構成 (2013)
  • Author(s): 竹下徹、柴田幸江、山下喜久
  • Organizer: 第86回日本細菌学会総会
  • Place of Presentation: 千葉市
• [Presentation] 歯周治療に伴う歯肉縁上プラークおよび唾液の細菌構成変化の比較 (2012)
  • Author(s): 竹下徹、山中渉、松尾和樹、柴田幸江、江島信興、山下喜久
  • Organizer: 第61回日本口腔衛生学会・総会
  • Place of Presentation: 横須賀市
• [Presentation] Bacterial community succession on a hydroxyapatite disk in oral cavity in caries-active and caries- free subjects (2012)
  • Author(s): T. Takeshita, M. Yasui, Y. Saeki, Y. Shibata, Y. Yamashita
  • Organizer: International symposium of Microbial Ecology 13
  • Place of Presentation: Copenhagen, Denmark
• [Presentation] Structural Shift of microbiota in dental plaque formed on a hydroxyapatite disk surface (2012)
  • Author(s): Y. Yamashita, T. Takeshita, M. Yasui, Y. Saeki, Y. Shibata, H, Ito
  • Organizer: vivo 6th ASM Conference on Biofilms
  • Place of Presentation: Miami, United States
• [Presentation] Bacterial community succession on a hydroxyapatite disk in oral cavity in caries-active and caries-free subjects. (2012)
  • Author(s): T. Takeshita
  • Organizer: International symposium of Microbial Ecology 13
  • Place of Presentation: Copenhagen, Denmark
• [Presentation] Structural Shift of microbiota in dental plaque formed on a hydroxyapatite disk surface in vivo (2012)
  • Author(s): Y. Yamashita
  • Organizer: 6th ASM Conference on Biofilms
  • Place of Presentation: Miami, United States
• [Presentation] Molecular analysis of bacterial community inhabiting in carious dentin (2011)
  • Author(s): J. Obata, T. Takeshita, M. Unemori, Y. Shibata, A. Akamine, Y. Yamashita
  • Organizer: 第59回 国際歯科研究学会日本部会(JADR)総会・学術大会
  • Place of Presentation: 広島市
  • Year and Date: 2011-10-08
• [Presentation] 口腔フローラの常在細菌構成バランスは口腔の健康状態と関連する (2011)
  • Author(s): 竹下徹
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岐阜市
  • Year and Date: 2011-10-02
• [Presentation] 硫化水素およびメチルメルカプタン高産生常在フローラの細菌構成 (2011)
  • Author(s): 竹下徹、鈴木奈央、中野善夫、米田雅裕、廣藤卓雄、山下喜久
  • Organizer: 第53回歯科基礎医学会学術大会・総会
  • Place of Presentation: 岐阜市
  • Year and Date: 2011-10-01
• [Presentation] Bacterial composition in saliva and its connection to oral biofilms (2011)
  • Author(s): Y. Yamashita, W. Yamanaka, T. Takeshita, K. Matsuo, T. Yokoyama
  • Organizer: Eurobiofilms 2011
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2011-07-07
• [Presentation] 口腔フローラの常在細菌構成バランスは口腔の健康状態と関連する (2011)
  • Author(s): 竹下徹
  • Organizer: 第53回歯科基礎医学会学術大会・総会(招待講演)
  • Place of Presentation: 岐阜市
• [Patent(Industrial Property Rights)] デンタルプラークの細菌叢の解析方法 (2012)
  • Inventor(s): 佐伯洋二、山下喜久、竹下徹、安井雅樹
  • Industrial Property Rights Holder: 佐伯洋二、山下喜久、竹下徹、安井雅樹
  • Industrial Property Rights Type: 特許
  • Filing Date: 2012-07-14