Analysis of calcium-signaling cascade in the regulation of spermfunction using gene-modified animals

• Project/Area Number: 23800038
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Laboratory animal science
• Research Institution: Osaka University
• Principal Investigator: MIYATA Haruhiko 大阪大学, 免疫学フロンティア研究センター, 特任助教 (50604732)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 受精 / 精子 / カルシウム

Research Abstract

I made a knockout(KO) mouse that lacks testis-specific calcineurin to understand its role in the regulation of sperm fertilizing ability. As a result, I found that calcineurin is important to maintain sperm motility and is necessary for fertilization. There were no problems found in the morphology of KO sperm. I also made a knockout mouse that lacks testis-specific calpain that is a calcium-dependent enzyme like calcineurin.

Research Products

• [Journal Article] Protease activation and the signal transduction pathway regulating motility in sperm from the water strider Aquarius remigis (2012)
  • Author(s): Miyata, H., Thaler, CD., Haimo, LT., Cardullo RA.
  • Journal Title: Cytoskeleton Volume: 69(4)巻 Pages: 207-220
  • Peer Reviewed
• [Journal Article] Protease activation and the signal transduction pathway regulating motility in sperm from the water strider Aquarius remigis (2012)
  • Author(s): Haruhiko Miyata, Catherine D.Thaler, Leah T.Haimo, Richard A.Cardullo
  • Journal Title: Cytoskeleton Volume: 69 Issue: 4 Pages: 207-220
  • DOI: 10.1002/cm.21012
  • Peer Reviewed
• [Presentation] 雌性生殖路を移行する精子のin vivoライブイメージング (2013)
  • Author(s): 宮田治彦
  • Organizer: 第3回vivid workshop
  • Place of Presentation: 石川県加賀市
  • Year and Date: 2013-02-22
• [Presentation] アメンボ精子の運動様式と運動開始調節機構 (2011)
  • Author(s): Miyata, H., Thaler, CD., Haimo, LT., Cardullo RA
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 神楽公民館(旭川市)
  • Year and Date: 2011-09-21
• [Presentation] アメンボ精子の運動様式と運動開始調節機構 (2011)
  • Author(s): Haruhiko Miyata, Catherine D.Thaler, Leah T.Haimo, Richard A.Cardullo
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 神楽公民館(旭川)(招待講演)
  • Year and Date: 2011-09-21
• [Remarks] 大阪大学微生物病研究所遺伝子機能解析分野ホームページ
  • URL: http://www.egr.biken.osaka-u.ac.jp/
• [Remarks] 大阪大学微生物病研究所遺伝子機能解析分野ホームページ
  • URL: http://www.egr.biken.osaka-u.ac.jp/