Identification of microRNA expression profile of cancer stem cells

• Project/Area Number: 23800039
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Tumor biology
• Research Institution: Osaka University
• Principal Investigator: HARAGUCHI Naotsugu 大阪大学, 医学系研究科, 助教 (30528609)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 癌幹細胞 / マイクロRNA / エクソゾーム / 情報ネットワーク / 骨髄

Research Abstract

In this study, we have identified specific microRNAs that regulate angiogenesis and histone modification in liver cancer stem cell (CSC) fraction. In colorectal cancer, we have also identified microRNA profiles of CSC fraction. These microRNAs were mainly involved in the secretary type and suggested to effect on surrounding microenvironmentfor cancer progression and metastasis.

Research Products

• [Journal Article] Depletion of JARID1B induces cellular senescence in human colorectal cancer (2013)
  • Author(s): Ohta K, Haraguchi N, Kano Y, Kagawa Y, Konno M, Nishikawa S, Hamabe A, Hasegawa S, Ogawa H, Fukusumi T, Uemura M, Nishimura J, Hata T, Takemasa I, Mizushima T, Noguchi Y, Ozaki M, Kudo T, Sakai D, Satoh T, Fukami M, Ishii M, Yamamot
  • Journal Title: Int J Oncol Volume: 42(4) Pages: 1212-8
• [Journal Article] Absence of CD71 transferrin receptor characterizes human gastric adenosquamous carcinoma stem cells (2012)
  • Author(s): Ohkuma M, Haraguchi N, Ishii H, Mimori K, Tanaka F, Kim HM, Shimomura M, Hirose H, Yanaga K, Mori M.
  • Journal Title: Ann Surg Oncol Volume: 19(4) Issue: 4 Pages: 1357-64
  • DOI: 10.1245/s10434-011-1739-7
  • Peer Reviewed
• [Journal Article] Reprogramming of gastrointestinal cancer cells (2012)
  • Author(s): Dewi D, Ishii H, Haraguchi N, Nishikawa S, Kano Y, Fukusumi T, Ohta K, Miyazaki S, Ozaki M, Sakai D, Satoh T, Nagano H, Doki Y, Mori M.
  • Journal Title: Cancer Sci Volume: 103(3) Issue: 3 Pages: 393-9
  • DOI: 10.1111/j.1349-7006.2011.02184.x
  • Peer Reviewed
• [Journal Article] Combination use of anti-CD133 antibody and SSA lectin can effectively enrich cells with high tumorigenicity (2011)
  • Author(s): MoriwakiK、Okudo K, Miyoshi E, et al
  • Journal Title: Cancer Science Volume: 102 Issue: 6 Pages: 1164-1170
  • DOI: 10.1111/j.1349-7006.2011.01923.x
  • NAID: 10029294388
  • Peer Reviewed
• [Journal Article] Cancer stem cell theory in gastrointestinal malignancies : recent progress and upcoming challenges (2011)
  • Author(s): 10. 1245/s10434-011-2040-5 14. Dewi DL, Ishii H, Kano Y, Nishikawa S, Haraguchi N, Sakai D, Satoh T, Doki Y, Mori M.
  • Journal Title: J Gastroenterol Volume: 46(10) Issue: 10 Pages: 1145-57
  • DOI: 10.1007/s00535-011-0442-6
  • Peer Reviewed
• [Journal Article] Increased CD13 Expression Reduces Reactive Oxygen Species, Promoting Survival of Liver Cancer Stem Cells via an Epithelial-Mesenchymal Transition-like Phenomenon (2011)
  • Author(s): Kim HM, Haraguchi N, Ishii H, Ohkuma M, Okano M, Mimori K, Eguchi H, Yamamoto H, Nagano H, Sekimoto M, Doki Y, Mori M.
  • Journal Title: Ann Surg Oncol Volume: 31 Issue: S3 Pages: 539-548
  • DOI: 10.1245/s10434-011-2040-5
  • Peer Reviewed
• [Journal Article] Reprogramming of Mouse and Human Cells to Pluripotency Using Mature MicroRNAs (2011)
  • Author(s): Miyoshi N, Ishii H, Nagano H, Haraguchi N, Dewi DL,Kano Y, Nishikawa S, Tanemura M, Mimori K, Tanaka F, Saito T, Nishimura J, Takemasa I, Mizushima T, Ikeda M, Yamamoto H, Sekimoto M, Doki Y, Mori M
  • Journal Title: Cell Stem Cell Volume: 8 Issue: 6 Pages: 633-8
  • DOI: 10.1016/j.stem.2011.05.001
  • Peer Reviewed
• [Journal Article] CD49f-positive cell population efficiently enriches colon cancer cancer-initiating cells
  • Author(s): Haraguchi N, Ishii H, Mimori K, OhtaK, Uemura M, Nishimura J, Hata T, Takemasa I, Mizushima T, Yamamoto H, Doki Y, Mori M
  • Journal Title: Int J Oncol Volume: (Accepted)
• [Presentation] 定期学術集会消化器癌における癌幹細胞の特性と標的化 (2013)
  • Organizer: 第113回日本外科学会
  • Place of Presentation: 福岡
  • Year and Date: 2013-04-10
• [Presentation] 肝臓癌幹細胞の分離・同定と治療標的化へ向けた検討 (2011)
  • Author(s): 原口直紹, 石井秀始, 永野浩昭, 土岐祐一郎, 森正樹
  • Organizer: 第21回日本サイトメトリー学会学術集会
  • Place of Presentation: 京都市国際交流会館
  • Year and Date: 2011-06-26
• [Presentation] 消化器癌における癌幹細胞の特性と標的化
  • Author(s): 原口 直紹
  • Organizer: 第113回 日本外科学会 定期学術集会
  • Place of Presentation: 福岡 国際会議場
• [Remarks]
  • URL: http://www.med.osaka-u.ac.jp/pub/gesurg/study/index.html
• [Remarks]
  • URL: http://www.med.osaka-u.ac.jp/pub/gesurg/study/index.html