• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The role of acetylation of histone variant H2AZ in chromatin dynamics upon DNA damage

Research Project

Project/Area Number 23810015
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Risk sciences of radiation/Chemicals
Research InstitutionKyoto University

Principal Investigator

MATSUDA Ryo  京都大学, 放射線生物研究センター, 研究員 (70614155)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
KeywordsTIP60 複合体 / ヒストン H2AX / ヒストン H2AZ / アセチル化 / DNA 損傷応答 / TIP60複合体 / ヒストンH2AX / ヒストンH2AZ / DNA損傷応答
Research Abstract

To understand the role of acetylation in chromatin dynamics upon DNA damage, we purified the DNA helicase TAP54, which is the component of the TIP60 histone acetyltransferase (HAT) complex, from nuclear extract of HeLa cells. We found that TAP54 complex has HAT activity. Mass spetrometry (MS) analysis indicated that histone acetyltranferase, which is different from TIP60, and histone variant H2AZ were identified in the TAP54 complex. Since we have already shown that TIP60 acetylates histone variant H2AX upon DNA damage, these findings suggest that the cross talk between acetylation of H2AX and H2AZ has important roles in DNA damage response.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • Research Products

    (9 results)

All 2013 2012 2011

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (8 results)

  • [Journal Article] The actin family member Arp6 and the histone variant H2A.Z are required for spatial positioning of chromatin in chicken cell nuclei.2012

    • Author(s)
      Maruyama E., Hori T., Tanabe H., Kitamura H., Matsuda R., Tone S., Pavel H., Habermann FA., Hase JV, Cremer C., Fukagawa T, and Harata(equal first authors)
    • Journal Title

      Journal of Cell Science

      Volume: 125 Pages: 3739-3743

    • DOI

      10.1242/jcs.103903

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Presentation] The role of chromatin dynamics in DNA damage-induced checkpoint activation2013

    • Author(s)
      Masae Ikura, Ryo Matsuda, Tsuyoshi Ikura
    • Organizer
      第3回 広島大学原爆放射線医科学研究所 第3回国際シンポジウム
    • Year and Date
      2013-02-12
    • Related Report
      2012 Final Research Report
  • [Presentation] The role of chromatin dynamics in DNA damage-induced checkpoint activation2013

    • Author(s)
      Tsuyoshi Ikura
    • Organizer
      第3回 広島大学原爆放射線医科学研究所 第3回国際シンポジウム
    • Place of Presentation
      広島大学霞キャンパス 広仁会館(広島県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒトアクチンファミリー遺伝子ノックアウト細胞を用いたヒト INO80 複合体のゲノム安定性維持および酸化ストレス応答における機能解析2012

    • Author(s)
      高橋裕一郎、松田涼、北村大志、西島仁、柴原慶一、原田昌彦
    • Organizer
      第35回分子生物学会年会
    • Year and Date
      2012-12-11
    • Related Report
      2012 Final Research Report
  • [Presentation] DNA 損傷応答におけるヒストン H2AX とH2AZ のアセチル化クロストーク2012

    • Author(s)
      松田涼、井倉正枝、原田昌彦、田代聡、井倉毅
    • Organizer
      放射線影響学会
    • Place of Presentation
      東北大学
    • Year and Date
      2012-09-07
    • Related Report
      2012 Final Research Report
  • [Presentation] DNA損傷応答におけるヒストンH2AXとH2AZのアセチル化クロストーク2012

    • Author(s)
      松田 涼
    • Organizer
      第55回放射線影響学会
    • Place of Presentation
      東北大学(宮城県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ヒトアクチンファミリー遺伝子ノックアウト細胞を用いたヒトINO80複合体のゲノム安定性維持および酸化ストレス応答における機能解析2012

    • Author(s)
      高橋 裕一郎
    • Organizer
      第35回分子生物学会年会
    • Place of Presentation
      福岡国際会議場・マリンメッセ福岡(福岡県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] The role of histone H2AX eviction in DNA damage-induced check point activation2011

    • Author(s)
      井倉毅
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒトhArp5およびhArp8遺伝子ノックアウト細胞を用いたINO80クロマチンリモデリング複合体の機能解析2011

    • Author(s)
      高橋裕一郎
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-15
    • Related Report
      2011 Annual Research Report

URL: 

Published: 2011-09-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi