| Project/Area Number |
23810015
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Kyoto University |
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Principal Investigator |
MATSUDA Ryo 京都大学, 放射線生物研究センター, 研究員 (70614155)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | TIP60 複合体 / ヒストン H2AX / ヒストン H2AZ / アセチル化 / DNA 損傷応答 / TIP60複合体 / ヒストンH2AX / ヒストンH2AZ / DNA損傷応答 |
|---|
| Research Abstract |
To understand the role of acetylation in chromatin dynamics upon DNA damage, we purified the DNA helicase TAP54, which is the component of the TIP60 histone acetyltransferase (HAT) complex, from nuclear extract of HeLa cells. We found that TAP54 complex has HAT activity. Mass spetrometry (MS) analysis indicated that histone acetyltranferase, which is different from TIP60, and histone variant H2AZ were identified in the TAP54 complex. Since we have already shown that TIP60 acetylates histone variant H2AX upon DNA damage, these findings suggest that the cross talk between acetylation of H2AX and H2AZ has important roles in DNA damage response.
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