Genome - wide analysis for PRC1 - bound RNA molecules .
| Project/Area Number |
23810035
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
System genome science
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
MASUI Osamu 独立行政法人理化学研究所, 免疫器官形成研究グループ, 研究員 (30579305)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ポリコーム転写抑制複合体 / RNA / RIP / エピジェネティクス / ポリコーム複合体 / PRC1/2 / Ring1B / ES細胞 / Xist / X染色体不活性化 / 細胞分化 |
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| Research Abstract |
Polycomb repressive complexes (PRC1 and PRC2) are important protein complexes, which carry out epigenetic gene silencing by adding post-translational modifications to Histone proteins. PRC1 and PRC2 have been proposed to execute their transcriptional silencing function through binding with RNA molecules. Using RNA immunoprecipitation combined with next-generation DNA sequencer (RIP-seq) against Ring1B protein, a component of PRC1, we have recently identified those RNA molecules in a genome-wide manner. Surprisingly, transcripts of many components of PRC1 are included in the Ring1B-bound RNA fractions. These results suggest that the amount of PRC components may be controlled by PRCs themselves, providing a fine-tuning mechanism to maintain constant expression level of PRCs.
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Report
(3 results)
Research Products
(9 results)
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[Journal Article] MicroRNA regulation of Cbx7 mediates a switch of Polycomb orthologs during ESC differentiation.2012
Author(s)
O'Loghlen A., Mu n oz - Cabello A.M., Gaspar - Maia A., Wu H.A., Banito A., Kunows ka N., Racek T., Pemberton H.N., Beolchi P., Lavial F., Masui O. , Vermeulen M., Carroll T., Graumann J., Heard E., Dillon N., Azuara V., Snijders A.P., Peters G., Bernstein E., Gil J.
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Journal Title
Cell Stem Cell.
Volume: vol. 6
Pages: 33-46
Related Report
Peer Reviewed
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