Genome - wide analysis for PRC1 - bound RNA molecules .

• Project/Area Number: 23810035
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: System genome science
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: MASUI Osamu 独立行政法人理化学研究所, 免疫器官形成研究グループ, 研究員 (30579305)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: ポリコーム転写抑制複合体 / RNA / RIP / エピジェネティクス / ポリコーム複合体 / PRC1/2 / Ring1B / ES細胞 / Xist / X染色体不活性化 / 細胞分化

Research Abstract

Polycomb repressive complexes (PRC1 and PRC2) are important protein complexes, which carry out epigenetic gene silencing by adding post-translational modifications to Histone proteins. PRC1 and PRC2 have been proposed to execute their transcriptional silencing function through binding with RNA molecules. Using RNA immunoprecipitation combined with next-generation DNA sequencer (RIP-seq) against Ring1B protein, a component of PRC1, we have recently identified those RNA molecules in a genome-wide manner. Surprisingly, transcripts of many components of PRC1 are included in the Ring1B-bound RNA fractions. These results suggest that the amount of PRC components may be controlled by PRCs themselves, providing a fine-tuning mechanism to maintain constant expression level of PRCs.

Research Products

• [Journal Article] MicroRNA regulation of Cbx7 mediates a switch of Polycomb orthologs during ESC differentiation. (2012)
  • Author(s): O'Loghlen A., Mu n oz - Cabello A.M., Gaspar - Maia A., Wu H.A., Banito A., Kunows ka N., Racek T., Pemberton H.N., Beolchi P., Lavial F., Masui O. , Vermeulen M., Carroll T., Graumann J., Heard E., Dillon N., Azuara V., Snijders A.P., Peters G., Bernstein E., Gil J.
  • Journal Title: Cell Stem Cell. Volume: vol. 6 Pages: 33-46
  • Peer Reviewed
• [Journal Article] ポリコーム複合体と染色体・核内構造 (2012)
  • Author(s): 増井 修、古関明彦
  • Journal Title: 細胞工学 Volume: vol. 31 Pages: 887-893
• [Journal Article] ポリコーム複合体と染色体・核内構造 (2012)
  • Author(s): 増井修
  • Journal Title: 細胞工学 Volume: ３１ Pages: 887-893
• [Journal Article] MicroRNA Regulation of Cbx7 Mediates a Switch of Polycomb Orthologs during ESC differentiation (2012)
  • Author(s): Ana O'Loghlen、増井修, 他
  • Journal Title: Cell Stem Cell Volume: Vol.10 Issue: 1 Pages: 33-46
  • DOI: 10.1016/j.stem.2011.12.004
  • Peer Reviewed
• [Journal Article] Live-Cell Chromosome Dynamics and Outcome of X Chromosome Pairing Events during ES Cell Differentiation (2011)
  • Author(s): 増井修, 他
  • Journal Title: Cell Volume: Vol.145 Issue: 3 Pages: 447-458
  • DOI: 10.1016/j.cell.2011.03.032
  • Peer Reviewed
• [Presentation] Live - cell imaging of X - chromosome inactivation in differentiating ES cells: visualisation of X - inactivation centre locus and Xist RNA (2012)
  • Author(s): 増井修
  • Organizer: 2012 Mouse Molecular Genetics Conference
  • Place of Presentation: Pacific Grove (USA)
• [Presentation] Live-cell imaging of X-chromosome inactivation in differentiating ES cells: visualisation of X-inactivation centre locus and Xist RNA (2012)
  • Author(s): Osamu Masui
  • Organizer: 2012 Mouse Molecular Genetics Conference
  • Place of Presentation: Pacific Grove, CA, USA
• [Remarks] 受賞: Genetics Society of America Poster Award. (1st position). Mouse Molecular Genetics Conference. 2012年10月6日、Pacific Grove (USA)
• [Remarks]
  • URL: http://first.lifesciencedb.jp/archives/2849