Development of a micromanipulator tracking brain micromotion in 3D for ultrastable intracellular recording.

• Project/Area Number: 23860071
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Dynamics/Control
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: OTA Keisuke 独立行政法人理化学研究所, 行動神経生理学研究チーム, 基礎科学特別研究員 (40610382)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 細胞内記録 / 行動神経生理学 / 脳振動計測 / 追従制御 / 高速画像処理 / 細胞内記録法

Research Abstract

Animal brains swags in vivo. On the other hand, the intracellular recording electrode is fastened on to a base in the experiment. The intracellular recording is necessary for elucidating the information processing in the brain, but the current intracellular recording system does not realize a long-term stable recording in behaving animals. To solve this problem, I will develop a micromanipulator tracking brain micromotion in 3D and aim to simultaneously record the membrane potentials from two neurons in behaving animals.