Analysis of function ofhuman epidermal growth factor receptor using yeast

• Project/Area Number: 23860074
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: FUKUDA NOBUO 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 研究員 (00613548)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 酵母フェロモン応答 / ヒト受容体 / 自己リン酸化 / 膜局在性

Outline of Final Research Achievements

In this study, we established a methodology to analyze function of human epidermal growth factor receptor (EGFR) by using yeast signal transduction as readout. EGFR is activated by autophosphorylation following dimerization. We succeeded in restoration of yeast signal transduction based on molecular interaction between constitutively activated EGFR and its adapter that recognizes and binds to the phosphorylated residue of EGFR. Our approach will provide a simple and easy tool for evaluation of EGFR inhibitors which are potential seed compounds for anticancer drugs.

Research Products

• [Journal Article] Artificial Conversion of the Mating-Type of Saccharomyces cerevisiae without Autopolyploidization (2013)
  • Author(s): Fukuda N, Matsukura S, Honda S
  • Journal Title: ACS Synthetic Biology Volume: (in press) Issue: 12 Pages: 697-704
  • DOI: 10.1021/sb400016j
  • Peer Reviewed
• [Journal Article] Transplantation of the GAL regulon into G-protein signaling circuitry in yeast. (2012)
  • Author(s): Ryo S, Ishii J, Iguchi Y, Fukuda N, Kondo A
  • Journal Title: Analytical Biochemistry Volume: vol.424 Issue: 1 Pages: 27-31
  • DOI: 10.1016/j.ab.2012.02.005
  • Peer Reviewed
• [Journal Article] Gγ recruitment system incorporating a novel signal amplification circuit to screen transient protein-protein interactions. (2011)
  • Author(s): Fukuda N, Ishii J, Kondo A
  • Journal Title: FEBS Journal Volume: vol.278 Issue: 17 Pages: 3086-3094
  • DOI: 10.1111/j.1742-4658.2011.08232.x
  • Peer Reviewed
• [Journal Article] Amplification of agonist stimulation of human G-protein-coupled receptor signaling in yeast. (2011)
  • Author(s): Fukuda N, Ishii J, Kaishima M, Kondo A
  • Journal Title: Analytical Biochemistry Volume: vol.417 Issue: 2 Pages: 182-187
  • DOI: 10.1016/j.ab.2011.06.006
  • Peer Reviewed
• [Presentation] Developing a screening method for desirably altering affinities of protein mutants based on yeastcellular signaling (2012)
  • Author(s): 海嶋 美里、 福田 展雄、石井 純、 近藤 昭彦
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場(福岡県)
  • Year and Date: 2012-12-14
• [Presentation] A new technology to screen desirably affinity-altered proteins based on yeast signal transductionmachinery (2012)
  • Author(s): 海嶋 美里、 福田 展雄、石井 純、近藤 昭彦
  • Organizer: YABEC2012
  • Place of Presentation: 徳島大学(徳島県)
  • Year and Date: 2012-10-27
• [Patent(Industrial Property Rights)] 自己倍数化抑制に基づく酵母の育種方法 (2013)
  • Inventor(s): 本田 真也, 福田 展雄, 松倉 智子
  • Industrial Property Rights Holder: 独立行政法人産業技術総合研究所
  • Industrial Property Number: 2013-014863
  • Filing Date: 2013-01-29