Intracellular dynamics of plant cell under cold conditions

• Project/Area Number: 23870002
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Utsunomiya University
• Principal Investigator: KODAMA Yutaka 宇都宮大学, バイオサイエンス教育研究センター, 助教 (00455213)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 植物細胞 / 低温応答 / 細胞小器官 / 蛍光イメージング / オルガネラ / 低温定位運動 / 葉緑体 / 蛍光タンパク質 / 遺伝子組換え

Research Abstract

To understand intracellular dynamics of plant cell under cold conditions, cold-induced organelle relocation movements were studied in the liverwort Marchantia polymorpha L. Our findings revealed that chloroplasts, nucleus and peroxisomes concurrently change their positions in the cell after cold treatments.

Research Products

• [Journal Article] Cold-induced organelle relocation in the liverwort Marchantia polymorpha L. Plant (2013)
  • Author(s): Ogasawara Y, Ishizaki K, Kohchi T and KodamaY
  • Journal Title: Cell& Environment Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Bimolecular fluorescence complementation analysis of protein-protein interaction: How to calculate signal-to-noise ratio (2013)
  • Author(s): Kodama Y and Hu CD
  • Journal Title: Methods in Cell Biology Volume: 113 Pages: 107-121
• [Journal Article] Bimolecular fluorescence complementation analysis of protein-protein interaction: How to calculate signal-to-noise ratio. (2013)
  • Author(s): Kodama Y & Hu CD
  • Journal Title: Methods in Cell Biology Volume: 113 Pages: 107-121
  • DOI: 10.1016/b978-0-12-407239-8.00006-9
  • ISBN: 9780124072398
• [Journal Article] Cold-induced organelle relocation in the liverwort Marchantia polymorpha L. (2013)
  • Author(s): Ogasawara Y, Ishizaki K, Kohchi T & Kodama Y
  • Journal Title: Plant, Cell & Environment Volume: 印刷中 Issue: 8 Pages: 1-9
  • DOI: 10.1111/pce.12085
  • Peer Reviewed
• [Journal Article] Bimolecular fluorescence complementation (BiFC): a 5-year update and future perspectives (2012)
  • Author(s): Kodama Y and Hu CD
  • Journal Title: BioTechniques Volume: 53 Pages: 285-298
  • Peer Reviewed
• [Journal Article] Bimolecular fluorescence complementation (BiFC): a 5-year update and future perspectives. (2012)
  • Author(s): Kodama Y & Hu CD
  • Journal Title: BioTechniques Volume: 53 Issue: 5 Pages: 285-298
  • DOI: 10.2144/000113943
  • Peer Reviewed
• [Presentation] 高輝度かつ正確なタンパク質間相互作用イメージングを目指した二分子蛍光補完法の改良 (2012)
  • Author(s): 児玉豊
  • Organizer: 第53回日本植物生理学会
  • Place of Presentation: 京都産業大学(招待講演)
  • Year and Date: 2012-03-16
• [Presentation] Organelle cryorelocation in Marchantia polymorpha L (2012)
  • Author(s): Kodama Y
  • Organizer: International Marchantia Workshop 2012 ・ Aso
  • Place of Presentation: Japan
• [Presentation] A rapid and simplified genetic transformation method for Marchantia polymorpha L (2012)
  • Author(s): Kodama Y
  • Organizer: International Marchantia Workshop 2012・Aso
  • Place of Presentation: Japan
• [Presentation] ゼニゴケを用いた細胞小器官の低温応答運動の解析 (2012)
  • Author(s): 小笠原有香、石崎公庸、河内孝之、児玉豊
  • Organizer: 第30回日本植物細胞分子生物学会大会
  • Place of Presentation: 生駒
• [Presentation] 高輝度かつ正確なタンパク質間相互作用イメージングを目指した二分子蛍光補完法の改良 (2012)
  • Author(s): 児玉豊
  • Organizer: 第53回日本植物生理学会・シンポジウム「最先端イメージングが拓く植物科学の新時代」
  • Place of Presentation: 京都
• [Presentation] 緑色二分子蛍光補完(BiFC)法の高輝度化 (2011)
  • Author(s): 児玉豊
  • Organizer: 第29回日本植物細胞分子生物学会
  • Place of Presentation: 九州大学
  • Year and Date: 2011-09-08
• [Presentation] 緑色二分子蛍光補完(BiFC)法の高輝度化 (2011)
  • Author(s): 児玉豊
  • Organizer: 第29回日本植物細胞分子生物学会大会
  • Place of Presentation: 福岡
• [Presentation] A rapid and simplified genetic transformation method for Marchantia polymorpha L.
  • Author(s): Yutaka Kodama
  • Organizer: International Marchantia Workshop 2012
  • Place of Presentation: Aso, Japan
  • Invited
• [Presentation] Organelle cryorelocation in Marchantia polymorpha L.
  • Author(s): Yutaka Kodama
  • Organizer: International Marchantia Workshop 2012
  • Place of Presentation: Aso, Japan
  • Invited
• [Presentation] 蛍光を用いたオルガネラ低温定位運動の解析
  • Author(s): 小笠原 有香、児玉 豊
  • Organizer: 第2回オプトバイオシンポジウム
  • Place of Presentation: 宇都宮
• [Presentation] 光合成生物に適した FRET イメージング技術の基盤開発
  • Author(s): 藤井 雄太、児玉 豊
  • Organizer: 第2回オプトバイオシンポジウム
  • Place of Presentation: 宇都宮
• [Presentation] 葉緑体の低温定位運動を制御する細胞骨格分子の同定
  • Author(s): 木村 俊、児玉 豊
  • Organizer: 第2回オプトバイオシンポジウム
  • Place of Presentation: 宇都宮
• [Remarks] 研究室ホームページ
  • URL: http://c-bio.mine.utsunomiya-u.ac.jp/lab/kodama.html
• [Remarks] 宇都宮大学研究拠点創成ユニット(UU-COE)
  • URL: http://c-bio.mine.utsunomiya-u.ac.jp/uu-coe/
• [Remarks] 宇都宮大学・バイオサイエンス教育研究センター・児玉研究室
  • URL: http://c-bio.mine.utsunomiya-u.ac.jp/lab/kodama/
• [Remarks] 宇都宮大学・研究拠点創成ユニット（UU-COE）
  • URL: http://c-bio.mine.utsunomiya-u.ac.jp/uu-coe/
• [Remarks]
  • URL: http://c-bio.mine.utsunomiya-u.ac.jp/lab/kodama.html