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Analysis of the cell division patterning during plant early embryogenesis by developing the optical manipulation techniques

Research Project

Project/Area Number 23870014
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Morphology/Structure
Research InstitutionNagoya University

Principal Investigator

KURIHARA Daisuke  名古屋大学, 理学研究科, 特任助教 (90609439)

Project Period (FY) 2011 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords植物胚発生 / 光顕微操作 / 細胞分裂 / 分裂期キナーゼ / 光操作
Research Abstract

Multicellular animals and plants develop from the single-celled zygote to form the mature embryo. During embryogenesis, the zygote follows a pattern of cell division to form the body plan of the embryo. In this study, I performed live-cell imaging using in vitroovule culture and the fluorescent marker lines in Arabidopsis thaliana. The data of live-cell imaging indicated that the timings of cell division are not strictly synchronized even in 4-cell embryo stage. Moreover, I established the gene induction system in a single-cell level in Arabidopsis embryo using the optical manipulation technique.

Report

(3 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • Research Products

    (26 results)

All 2013 2012 2011 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (16 results) (of which Invited: 1 results) Remarks (3 results)

  • [Journal Article] Live-cell analysis of plant reproduction: Live-cell imaging, optical manipulation, and advanced microscopy technologies2013

    • Author(s)
      Kurihara D., Hamamura Y., Higashiyama T.
    • Journal Title

      Dev Growth Differ

      Volume: 55 Issue: 4 Pages: 462-473

    • DOI

      10.1111/dgd.12040

    • NAID

      40019735095

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis ASYMMETRICLEAVES2 protein required for leaf morphogenesis consistently forms speckles during mitosis of tobacco BY-2 cells via signals in its specific sequence.2012

    • Author(s)
      Luo, L., et al.
    • Journal Title

      J. Plant Res.

      Volume: 125 Issue: 5 Pages: 661-668

    • DOI

      10.1007/s10265-012-0479-5

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Live-Cell Imaging of Double Fertilization and Embryogenesis in Plants2012

    • Author(s)
      Kurihara D., Hamamura Y., Higashiyama T
    • Journal Title

      Conference Proceedings APMC 10 / ICONN 2012 / ACMM 22

      Volume: 1055 Pages: 1-2

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Live-Cell Imaging of Double Fertilization and Embryogenesis in Plants2012

    • Author(s)
      栗原大輔
    • Journal Title

      Conference Proceedings APMC 10/ICONN 2012/ACMM 22

      Volume: 1055 Pages: 1-2

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Programmed induction of endoreduplication by DNA double-strand breaks in Arabidopsis2011

    • Author(s)
      Adachi, S.
    • Journal Title

      Proc.Natl.Acad.Sci., USA

      Volume: 108 Issue: 24 Pages: 10004-10009

    • DOI

      10.1073/pnas.1103584108

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] 細胞分裂期において分裂期キナーゼが制御する染色体動態2011

    • Author(s)
      栗原大輔
    • Journal Title

      Plant Morphology

      Volume: 23 Pages: 81-89

    • NAID

      130002152901

    • URL

      http://dx.doi.org/10.5685/plmorphol.23.81

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and characterization of plant Haspin kinase as a histone H3 threonine kinase2011

    • Author(s)
      Kurihara, D., Matsunaga, S., et al
    • Journal Title

      BMC Plant Biol

      Volume: 11 Issue: 1 Pages: 73-73

    • DOI

      10.1186/1471-2229-11-73

    • NAID

      120006995017

    • Related Report
      2012 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Presentation] 組織深部のライブイメージングと光細胞操作で花の内部を探る2012

    • Author(s)
      東山哲也, 栗原大輔
    • Organizer
      日本女子大学バイオイメージングセンター第4回公開シンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2012-10-27
    • Related Report
      2012 Final Research Report
  • [Presentation] 光顕微操作とライブイメージングで迫るパターン形成2012

    • Author(s)
      栗原大輔, 牛王啓太, 東山哲也
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      兵庫
    • Year and Date
      2012-09-17
    • Related Report
      2012 Final Research Report
  • [Presentation] ホロニックコミュニケーションを担うシグナリング分子の可視化に向けて2012

    • Author(s)
      栗原大輔, 東山哲也
    • Organizer
      日本植物形態学会第24回総会・大会
    • Place of Presentation
      兵庫
    • Year and Date
      2012-09-14
    • Related Report
      2012 Final Research Report
  • [Presentation] 光顕微操作とライブイメージングで迫る植物胚発生2012

    • Author(s)
      栗原大輔
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都産業大学
    • Year and Date
      2012-03-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] 光顕微操作とライブイメージングで迫る植物胚発生2012

    • Author(s)
      栗原大輔
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-16
    • Related Report
      2012 Final Research Report
  • [Presentation] Live-Cell Imaging of Double Fertilization and Embryogenesis in Plants, APMC 10 / ICONN 2012 / ACMM 222012

    • Author(s)
      Kurihara D., Hamamura Y., Higashiyama T
    • Organizer
      Perth
    • Place of Presentation
      Australia
    • Year and Date
      2012-02-08
    • Related Report
      2012 Final Research Report
  • [Presentation] Live-Cell Imaging of Double Fertilization and Embryogenesis in Plants2012

    • Author(s)
      栗原大輔
    • Organizer
      the 10th Asia-Pacific Microscopy Conference (APMC 10), the 2012 International Conference on Nanoscience and Nanotechnology (ICONN 2012) and the 22nd Australian Conference on Microscopy and Microanalysis (ACMM 22)
    • Place of Presentation
      Perth Convention and Exhibition Centre, Western Australia, Australia(招待講演)
    • Year and Date
      2012-02-08
    • Related Report
      2011 Annual Research Report
  • [Presentation] シロイヌナズナ胚発生過程のライブイメージング2011

    • Author(s)
      栗原大輔, 牛王啓太, 東山哲也
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京
    • Year and Date
      2011-09-18
    • Related Report
      2012 Final Research Report
  • [Presentation] シロイヌナズナ胚発生過程のライブイメージング2011

    • Author(s)
      栗原大輔
    • Organizer
      日本植物学会第75回大会
    • Place of Presentation
      東京大学駒場キャンパス
    • Year and Date
      2011-09-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] 光操作技術によるシロイヌナズナ胚発生過程の解析2011

    • Author(s)
      栗原大輔, 牛王啓太, 東山哲也
    • Organizer
      日本植物形態学会第23回総会・大会
    • Place of Presentation
      東京
    • Year and Date
      2011-09-16
    • Related Report
      2012 Final Research Report
  • [Presentation] 光操作技術によるシロイヌナズナ胚発生過程の解析2011

    • Author(s)
      栗原大輔
    • Organizer
      日本植物形態学会第23回総会・大会
    • Place of Presentation
      日本女子大学目白キャンパス
    • Year and Date
      2011-09-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] Live-cell imaging of embryo development in Arabidopsis thaliana2011

    • Author(s)
      栗原大輔, 東山哲也
    • Organizer
      第63回日本細胞生物学会大会
    • Place of Presentation
      北海道
    • Year and Date
      2011-06-27
    • Related Report
      2012 Final Research Report
  • [Presentation] Live-cell imaging of embryo development in Arabidopsis thaliana2011

    • Author(s)
      栗原大輔
    • Organizer
      第63回日本細胞生物学会大会
    • Place of Presentation
      北海道大学
    • Year and Date
      2011-06-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] ホロニックコミュニケーションを担うシグナリング分子の可視化に向けて

    • Author(s)
      栗原大輔
    • Organizer
      日本植物形態学会第24回総会・大会
    • Place of Presentation
      兵庫県立大学書写キャンパス(兵庫県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 光顕微操作とライブイメージングで迫るパターン形成

    • Author(s)
      栗原大輔
    • Organizer
      日本植物学会第76回大会
    • Place of Presentation
      兵庫県立大学書写キャンパス(兵庫県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 組織深部のライブイメージングと光細胞操作で花の内部を探る

    • Author(s)
      栗原大輔
    • Organizer
      日本女子大学バイオイメージングセンター 第4回公開シンポジウム
    • Place of Presentation
      日本女子大学八十年館(東京都)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Remarks]

    • URL

      http://www.liveholonics.com

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.bio.nagoya-u.ac.jp/paper/2011-17/07.html

    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.bio.nagoya-u.ac.jp/thesis/2011-17/07.html

    • Related Report
      2011 Annual Research Report

URL: 

Published: 2011-09-05   Modified: 2019-07-29  

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