Analysis of the cell division patterning during plant early embryogenesis by developing the optical manipulation techniques
| Project/Area Number |
23870014
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Morphology/Structure
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| Research Institution | Nagoya University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 植物胚発生 / 光顕微操作 / 細胞分裂 / 分裂期キナーゼ / 光操作 |
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| Research Abstract |
Multicellular animals and plants develop from the single-celled zygote to form the mature embryo. During embryogenesis, the zygote follows a pattern of cell division to form the body plan of the embryo. In this study, I performed live-cell imaging using in vitroovule culture and the fluorescent marker lines in Arabidopsis thaliana. The data of live-cell imaging indicated that the timings of cell division are not strictly synchronized even in 4-cell embryo stage. Moreover, I established the gene induction system in a single-cell level in Arabidopsis embryo using the optical manipulation technique.
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Report
(3 results)
Research Products
(26 results)
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[Presentation] Live-Cell Imaging of Double Fertilization and Embryogenesis in Plants2012
Author(s)
栗原大輔
Organizer
the 10th Asia-Pacific Microscopy Conference (APMC 10), the 2012 International Conference on Nanoscience and Nanotechnology (ICONN 2012) and the 22nd Australian Conference on Microscopy and Microanalysis (ACMM 22)
Place of Presentation
Perth Convention and Exhibition Centre, Western Australia, Australia(招待講演)
Year and Date
2012-02-08
Related Report
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