Structure based design of novel RNA aptamer against AML1
| Project/Area Number |
23870026
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
NOMURA Yusuke 東京理科大学, 理学部・応用化学科, 助教 (10609308)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | RNA アプタマー / 立体構造 / RNAアプタマー / AML / SELEX / 白血病原因タンパク質 / 核酸-蛋白質相互作用 |
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| Research Abstract |
The purpose of this study is to obtain a novel RNA aptamer against AML1 Runt domain by the structure based design.
(1)The designed aptamer, Apt14 has high affinity for the Runt domain.
(2) The mutant analysis of the Apt14 suggested that internal loop and terminal stem structure are important for the affinity to the Runt domain.
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Report
(3 results)
Research Products
(10 results)
