| Project/Area Number |
23870027
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Tokyo University of Science |
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Principal Investigator |
TOSHIMA Junko 東京理科大学, 総合研究機構, ポストドクトラル研究員 (00431552)
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|---|
| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | エンドサイトーシス / 膜輸送 / アクチン / 小胞輸送 / エンドソーム / クラスリン / 細胞内輸送 |
|---|
| Research Abstract |
To elucidate the mechanisms how activated receptor is recruited to clathrin coated pits, we screened yeast genome library, and identified Bmh2p, yeast homologue of 14-3-3 protein, as a binding protein for yeast GPCR. In addition, we tagged 13 human GPCR with GFP and succeeded in expressing in yeast cells. We also performed screening of about 80 % of yeast gene knock out strains for endocytosis defect, and identified about 200 genes that exhibited defects in endocytic transport.
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