Investigation of ATP regulation mechanisms during photo-acclimation in plants

• Project/Area Number: 23870033
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: TOKUTSU Ryutaro 基礎生物学研究所, 環境光生物学研究部門, 助教 (60613940)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 光合成 / 環境適応 / エネルギー生産 / 色素体機能・光合成 / 環境応答 / バイオイメージング

Research Abstract

In this study, I tried to investigate the ATP regulation mechanisms during photo-acclimation in the green photosynthetic alga Chlamydomonas reinhardtii. The protein ATeam, the molecular fluorescence probe for visualizing the ATP concentration in the living cell, was managed to install into the C. reinhardtii. Here, I finally succeeded to obtain the ATeam transgenic C. reinhardtii, and the cell showed functional FRET signals due to the modification of intracellular ATP concentration during environmental changes.

Research Products

• [Journal Article] A dual strategy to cope with high light in Chlamydomonas reinhardtii (2013)
  • Author(s): *Allorent, G., *Tokutsu, R. , Roach, T., Peers, G., Cardol, P., Girard-Bascou, J., Seigneurin-Berny, D., Petroutsos, D., Kuntz, M., Breyton, C., Franck, F., Wollman, F. A., Niyogi, K. K., Krieger-Liszkay, A., Minagawa, J., and Finazzi, G.
  • Journal Title: The Plant Cell Volume: 25 Pages: 545-557
  • Peer Reviewed
• [Journal Article] Revisiting the supramolecular organization of photosystem II in Chlamydomonas reinhardtii (2012)
  • Author(s): Tokutsu, R., Kato, N., Bui, K. H., Ishikawa, T., and Minagawa, J
  • Journal Title: J B i o l C h e m 287 Volume: 287 Pages: 31574-31581
  • Peer Reviewed
• [Journal Article] Revisiting the supramolecular organization of photosystem II in Chlamydomonas reinhardtii (2012)
  • Author(s): Ryutaro Tokutsu
  • Journal Title: Journal of Biological Chemistry Volume: 287 Issue: 37 Pages: 31574-31581
  • DOI: 10.1074/jbc.m111.331991
  • Peer Reviewed
• [Presentation] Guillaume Allorent 、 Giovanni Finazzi、皆川純, 緑藻の複合的強 光適応戦略 (2013)
  • Author(s): 得津隆太郎
  • Organizer: 第54回日本植物生理学会
  • Place of Presentation: 岡山大学 日本
• [Presentation] 緑藻の複合的強光適応戦略 (2013)
  • Author(s): 得津 隆太郎
  • Organizer: 第54回 日本植物生理学会
  • Place of Presentation: 岡山大学, 日本
• [Presentation] 緑藻のNPQに関与する光化学系超複合体の精製 (2012)
  • Author(s): 得津隆太郎、皆川純
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都府)
  • Year and Date: 2012-03-16
• [Presentation] Formation of PSII-LHCII-LHCSR supercomplex under NPQ inducible conditions (2012)
  • Author(s): R.Tokutsu. and J. Minagawa
  • Organizer: 15th International Conference on the Cell and Molecular Biology of Chlamydomonas
  • Place of Presentation: Chlamydomonas. Potsdam, Germany
• [Presentation] Formation of PSII-LHCII-LHCSR supercomplex under NPQ inducible conditions (2012)
  • Author(s): Ryutaro Tokutsu
  • Organizer: 15th International Conference on the Cell and Molecular Biology of Chlamydomonas
  • Place of Presentation: Potsdam, Germany
• [Remarks] ホームページ等 緑藻は二重の強光馴化により光合成器官をまもっている
  • URL: http://www.nibb.ac.jp/pressroom/news/2013/03/15.html
• [Remarks] 研究成果論文が科学誌に受理されました (2012-01)
  • URL: http://chlamynibb.blogspot.jp/2012/07/blog-post.html
• [Remarks] 研究成果論文が科学誌に受理されました (2013-01)
  • URL: http://chlamynibb.blogspot.jp/2013_02_01_archive.html
• [Remarks] 緑藻は二重の強光馴化により光合成器官をまもっている
  • URL: http://www.nibb.ac.jp/pressroom/news/2013/03/15.html
• [Remarks] 研究成果論文が科学誌に受理されました（2012-01）
  • URL: http://chlamynibb.blogspot.jp/2012/07/blog-post.html
• [Remarks] 研究成果論文が科学誌に受理されました（2013-01）
  • URL: http://chlamynibb.blogspot.jp/2013_02_01_archive.html