Functional analysis of the sex-determining gene in Oryzias dancena using transgenic techniques
| Project/Area Number |
23870036
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Developmental biology
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| Research Institution | National Institute for Basic Biology |
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Principal Investigator |
TAKEHANA Yusuke 基礎生物学研究所, バイオリソース研究室, 助教 (60432093)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 性染色体 / 性決定遺伝子 / 遺伝子導入 |
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| Research Abstract |
In this study, we identified the sex-determining locus of Oryzias dancena by positional cloning, and found that this region on the Y chromosome contains an enhancer that upregulates the Sox3 specifically in gonads. Sex reversed phenotypes in transgenic fish and zinc-finger nuclease-induced mutants of the Y chromosomal Sox3 allele all point its critical role in sex determination, suggesting that the neo-Y chromosome of O. dancena arose by co-option of Sox3
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Report
(3 results)
Research Products
(18 results)
