Functional analysis of the sex-determining gene in Oryzias dancena using transgenic techniques

• Project/Area Number: 23870036
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: TAKEHANA Yusuke 基礎生物学研究所, バイオリソース研究室, 助教 (60432093)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 性染色体 / 性決定遺伝子 / 遺伝子導入

Research Abstract

In this study, we identified the sex-determining locus of Oryzias dancena by positional cloning, and found that this region on the Y chromosome contains an enhancer that upregulates the Sox3 specifically in gonads. Sex reversed phenotypes in transgenic fish and zinc-finger nuclease-induced mutants of the Y chromosomal Sox3 allele all point its critical role in sex determination, suggesting that the neo-Y chromosome of O. dancena arose by co-option of Sox3

Research Products

• [Journal Article] Molecular cloning and hybridization of the repetitive DNA sequences that comprise constitutive heterochromatin of the W chromosomes of medaka fishes (2012)
  • Author(s): Y.Takehana, K.Naruse, et al.
  • Journal Title: Chromosome research Volume: 20 Issue: 1 Pages: 71-81
  • DOI: 10.1007/s10577-011-9259-7
  • Peer Reviewed
• [Journal Article] An autosomal locus controls sex reversal in interspecific nybrids of the medaka fishes (2011)
  • Author(s): M.Kato, Y.Takehana, et al.
  • Journal Title: Heredity Volume: 107 Issue: 6 Pages: 523-529
  • DOI: 10.1038/hdy.2011.38
  • Peer Reviewed
• [Presentation] Evolution of the neo-Y chromosome through co-option of Sox3 in a medaka-related fish. (2013)
  • Author(s): Yusuke Takehana
  • Organizer: 8th European Zebrafish Meeting
  • Place of Presentation: The Palau de Congressos de Catalunya (Barcelona, Spain)
• [Presentation] Molecular phylogeny of the medaka fishes. A mini-symposium (2012)
  • Author(s): Takehana Y
  • Organizer: "What is medaka? (from the biological point of view)"
  • Place of Presentation: Nagoya, Aichi, Japan
  • Year and Date: 2012-01-29
• [Presentation] Molecular phylogeny of the medaka fishes (2012)
  • Author(s): Takehana Y
  • Organizer: A mini-symposium, "What is medaka? (from the biological point of view)"
  • Place of Presentation: Nagoya Higashiyama Zoo(愛知県)
  • Year and Date: 2012-01-29
• [Presentation] インドメダカ性決定遺伝子の同定 (2012)
  • Author(s): 竹花佑介
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪大学(大阪)
• [Presentation] Evolution of diverse sex determination mechanisms in the medaka fishes (2012)
  • Author(s): Takehana Y
  • Organizer: 7th International Conference on Stickleback Behavior and Evolution
  • Place of Presentation: Seattle, USA
• [Presentation] Independent evolution of sex chromosomes through recurrent use of Sox3 in a medaka fish and mammals (2012)
  • Author(s): Takehana Y
  • Organizer: The 58th/60th NIBB Conference, Germline -Specification, Sex, and Stem Cells-
  • Place of Presentation: Okazaki,Aichi, Japan
• [Presentation] Different origin of sex chromosomes in the medaka fishes (2012)
  • Author(s): Takehana Y, Naruse K, Hamaguchi S, Sakaizumi M
  • Organizer: The 8th Okazaki Biology Conference
  • Place of Presentation: Okazaki,Aichi, Japan
• [Presentation] Independent evolution of sex chromosomes through recurrent use of Sox3 in a medaka fish and mammals. (2012)
  • Author(s): Yusuke Takehana
  • Organizer: The 58th/60th NIBB Conference, Germline -Specification, Sex, and Stem Cells-
  • Place of Presentation: 岡崎コンファレンスセンター（愛知県岡崎市）
• [Presentation] Evolution of diverse sex determination mechanisms in the medaka fishes. (2012)
  • Author(s): Yusuke Takehana
  • Organizer: 7th International Conference on Stickleback Behavior and Evolution
  • Place of Presentation: Islandwood (Seattle, USA)
• [Presentation] インドメダカ性決定 遺伝子の同定 (2012)
  • Author(s): 竹花 佑介
  • Organizer: 日本動物学会第83回大会
  • Place of Presentation: 大阪大学（大阪）
• [Presentation] Different origin of sex chromosomes in the medaka fishes (2012)
  • Author(s): Takehana Y, Naruse K, Hamaguchi S, Sakaizumi M
  • Organizer: The 8th Okazaki Biology Conference
  • Place of Presentation: Okazaki Conference Center(愛知県)
• [Presentation] Turnover of sex chromosomes in the medaka fishes (2011)
  • Author(s): Takehana Y, Naruse K, Hamaguchi S, Sakaizumi M
  • Organizer: National BioResource Project Medaka, The 1st Strategic Meeting for Medaka Research
  • Place of Presentation: Okazaki,Aichi, Japan
• [Presentation] メダカ属魚類における新規性決定遺伝子の進化機構 (2011)
  • Author(s): 竹花佑介
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川市大雪クリスタルホール(北海道旭川市)
• [Presentation] Turnover of sex chromosomes in the medaka fishes (2011)
  • Author(s): Takehana Y, Naruse K, Hamaguchi S, Sakaizumi M
  • Organizer: National BioResource Project Medaka, The 1st Strategic Meeting for Medaka Research
  • Place of Presentation: Okazaki Conference Center(愛知県)
• [Presentation] メダカ属魚類における新規性決定遺伝子の進化機構 (2011)
  • Author(s): 竹花佑介
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川市大雪クリスタルホール(北海道)
• [Book] In Genome Mapping and Genomics in Laboratory Animals, P (2012)
  • Author(s): Kimura T, Kamei Y, Takehana Y, Sasado T, Naruse K
  • Publisher: Springer. Medaka genomics and the methods and resources for decoding genomic functions