Establishment of neuronal cell lines derived from goat hypothalamus and analysis of reproductive mechanisms in domestic animals
| Project/Area Number |
23880012
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Basic veterinary science/Basic zootechnical science
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| Research Institution | Nagoya University |
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Principal Investigator |
MATSUDA Fuko 名古屋大学, 大学院・生命農学研究科, 准教授 (10608855)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 畜産学 / 応用動物 / 神経科学 / 細胞・組織 |
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| Research Abstract |
To analyze the neuroendocrine system that regulate reproduction in domestic ruminants by in vitro methods, we tried to establish new cell lines derived from goat hypothalamic neurons. We immortalized goat hypothalamic primary cultured cells and obtained cell clones derived from neurons. Among the hypothalamic neuronal cell clones, we determined multiple cell lines that express kisspeptin gene and neurokinin gene. We further examined the transcriptional regulation mechanism of goat neurokinin gene and determined both transcriptional activation and repression domain of goat neurokinin gene 5`-upstream region.
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Report
(3 results)
Research Products
(41 results)
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[Presentation] Kisspeptin neurons in the medial preoptic area of castrated male goats mediate the surge-like luteinizing hormone secretion induced by acute elevations in circulating estrogen2012
Author(s)
Nakatsukasa K, Suetomi Y, Naniwa Y, Ito D, Inoue N, Wakabayashi Y, Okamura H, Maeda K-I, Uenoyama Y, Tsukamura H, Matsuda F, Ohkura S
Organizer
The 2nd World Conference on Kisspeptin Signaling in the Brain
Place of Presentation
東京
Related Report
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[Presentation] Comparison of the efficacy of intravenous treatment of kisspeptin and GnRH on ovarian activity in Japanese Black beef cows2012
Author(s)
Naniwa Y, Nakatsukasa K, Setsuda S, Oishi S, Fujii N, Wakabayashi Y, Okamura H, Uenoyama Y, Tsukamura H, Maeda K-I, Matsuda F, Ohkura S
Organizer
The 2nd World Conference on Kisspeptin Signaling in the Brain
Place of Presentation
東京
Related Report
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[Presentation] Kisspeptin stimulates luteinizing hormone secretion and follicular development in Japanese Black beef cows2011
Author(s)
Naniwa Y, Nakatsukasa K, Setsuda S, Oishi S, Fujii N, Wakabayashi Y, Okamura H, Matsuda F, Uenoyama Y, Tsukamura H, Maeda K-I, Ohkura S
Organizer
2nd World Congress on Reproductive Biology
Place of Presentation
ケアンズコンベンションセンター(オーストラリア)
Year and Date
2011-10-10
Related Report
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[Presentation] Kisspeptin stimulates luteinizing hormone secretion and follicular development in Japanese Black beef cows2011
Author(s)
Naniwa Y, Nakatsukasa K, Setsuda S, Oishi S, Fujii N, Wakabayashi Y, Okamura H, Matsuda F, Uenoyama Y, Tsukamura H, Maeda K-I, Ohkura S.
Organizer
2nd World Congress on Reproductive Biology
Place of Presentation
ケアンズ、オーストラリア
Related Report
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