Research of nitrogen metabolism controlling system in insects
| Project/Area Number |
23880014
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
YOSHINAGA Naoko 京都大学, 大学院・農学研究科, 助教 (40456819)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 鱗翅目幼虫 / FACs / 窒素代謝 / アミノ酸 / 腸管 / Orbitrap |
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| Research Abstract |
Fatty acid amino acid conjugates (FACs) in lepidopteran caterpillars enhance their nitrogen assimilation and are considered to accelerate their growth ratio. The research focused on the biosynthetic mechanism of FACs in lepidopteran caterpillars.
For the first time, in vitro biosynthesis of glutamate conjugates were observed when the substrates were incubated with a midgut tissue of Spodoptera litura, silkworm, tobacco hornworm and a few other species. That means glutamate is directly conjugated with fatty acid in lepidopteran caterpillars, suggesting the synthetic routeis the same with the case of fruit fly Drosophila melanogaster but clearly different from the case of crickets Gryllus emma. Crickets first conjugate glutamine with fatty acid and modify the side chain of glutamine moiety to make a glutamate conjugate. Although FACs in these insects share a common ground such as localization in themidgut tissues and the same analog pattern consisting of glutamine/glutamate, the finding suggest that at least glutamate type FACs were independently developed in these insect order.
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Report
(3 results)
Research Products
(19 results)
