| Project/Area Number |
23880016
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied biochemistry
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| Research Institution | Kobe University |
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Principal Investigator |
TSURUTA Sachiko 神戸大学, 大学院・農学研究科, 農学研究科研究員 (30608625)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 好冷菌 / 低温活性酵素 / 糖転移酵素 / 糖鎖 |
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| Research Abstract |
The aim of this study is construction of one-batch system for glycan synthesis using enzymes showing different optimal temperature. To achieve the goal, we first cloned 6 genes of glycosyltransferase, group 2 family proteins (GT-2) from Shewanella frigidimarina, and constructed expression system of GT-2 proteins. As a result, we obtained two soluble recombinant GT-2s, using the expression vector derived from pCold I. Surveying the protein structure by X-ray analysis will enables us to clarify “structural factors” for eliciting cold-activity. Based on the results, we will create new types of GT-2 enzymes with cold-activity.
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