Investigating the establishment of cell culture from oyster hepatopancreas tissues for the concentration of norovirus

Research Project

Project/Area Number	23880026
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Applied microbiology
Research Institution	Fukuoka Women's University
Principal Investigator	KOBAYASHI Hiroshi 福岡女子大学, 国際文理学部, 講師 (00610255)
Project Period (FY)	2011 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000) Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	ノロウイルス / カキ細胞培養 / 濃縮 / 食中毒 / 濃縮法 / 検出法 / 細胞培養 / カキ中腸線
Research Abstract	Cell culture from oyster hepatopancreas tissue was examined for the development of concentration method of norovirus． The cells derived from oyster hepatopancereas tissue survived and grew well in LDF medium (50%D-MEM, 35% F-12, 15% L-15) supplemented with 10% FBS, 10μg/ml insulin, 10 nM sodium selenite, 5.5μg/ml Transferrin, 2 mM GlutaMax，0．1x MEM-non essential amino acid, and 50 ng/ml FGF or EGF．