| Project/Area Number |
23880026
|
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
|---|
| Research Field |
Applied microbiology
|
|---|
| Research Institution | Fukuoka Women's University |
|---|
Principal Investigator |
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| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
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| Keywords | ノロウイルス / カキ細胞培養 / 濃縮 / 食中毒 / 濃縮法 / 検出法 / 細胞培養 / カキ中腸線 |
|---|
| Research Abstract |
Cell culture from oyster hepatopancreas tissue was examined for the development of concentration method of norovirus． The cells derived from oyster hepatopancereas tissue survived and grew well in LDF medium (50%D-MEM, 35% F-12, 15% L-15) supplemented with 10% FBS, 10μg/ml insulin, 10 nM sodium selenite, 5.5μg/ml Transferrin, 2 mM GlutaMax，0．1x MEM-non essential amino acid, and 50 ng/ml FGF or EGF．
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