Dynamics of microtubules and plasma membrane domain during secondary cell wall deposition

• Project/Area Number: 23880029
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Wood science
• Research Institution: Forestry and Forest Products Research Institute
• Principal Investigator: TAKATA Naoki 独立行政法人森林総合研究所, 森林バイオ研究センター, 研究員 (90605544)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: セルロースミクロフィブリル / 細胞壁 / 表層微小管 / ポプラ / ライブセルイメージング / ミクロフィブリル / マイクロドメイン

Research Abstract

Orientation of cellulose microfibril deposition is controlled by cortical microtubules. The aim of this study is to reveal dynamics of cortical microtubules during the secondary cell wall deposition. We generated transgenic aspens in which microtubules were labeled by GFP expression. Cortical microtubules were visualized by GFP fluorescence in leaf, root and phloem cells in the transgenic plants. However, GFP fluorescence was weak in xylem fiber cells due to low expression of GFP-tagged protein.

Research Products

• [Journal Article] ドイツで開催された国際会議(Plant Biology Congress Freiburg2012)の概要 (2013)
  • Author(s): 高田直樹
  • Journal Title: 森林遺伝育種 Volume: 2 Pages: 37-40
  • NAID: 130007872726
  • URL: http://fgtb.ac.affrc.go.jp/publish/FGTB_ISSN_2187-350X/Vol.2/FGTB_Vol2_No.1/FGTB_V2N1_topic4.pdf
• [Journal Article] ドイツで開催された国際会議（Plant Biology Congress Freiburg 2012）の概要. (2013)
  • Author(s): 高田直樹
  • Journal Title: 森林遺伝育種 Volume: 2 Pages: 37-40
  • NAID: 130007872726
• [Journal Article] A simple and efficient transient transformation for hybrid aspen (Populus tremula × P. tremuloides) (2012)
  • Author(s): Takata N, Eriksson ME
  • Journal Title: Plant Methods Volume: 8 Issue: 1 Pages: 30-30
  • DOI: 10.1186/1746-4811-8-30
  • Peer Reviewed
• [Journal Article] モデル樹木・ポプラの生物情報データベースの現状と活用 (2011)
  • Author(s): 高田直樹
  • Journal Title: 林木の育種 Volume: 241 Pages: 27-30
  • NAID: 40019039140
• [Presentation] 全ゲノム重複後の重複遺伝子対の発現変化 -ポプラ・セルロース合成酵素の機能分化- (2013)
  • Author(s): 高田直樹, 谷口亨
  • Organizer: 第63回日本木材学会
  • Place of Presentation: 岩手大学(盛岡)
  • Year and Date: 2013-03-27
• [Presentation] Molecular Phylogeny and Structural Divergence of the EPF/EPFL Gene Family (2013)
  • Author(s): Takata N, Yokota K, Ohki S, Mori M, Taniguchi T, Kurita M
  • Organizer: 第54回日本植物生理学会
  • Place of Presentation: 岡山大学(岡山)
  • Year and Date: 2013-03-23
• [Presentation] Molecular Phylogeny and Structural Divergence of the EPF/EPFL Gene Family. (2013)
  • Author(s): Takata N, Yokota K, Ohki S, Mori M, Taniguchi T, Kurita M
  • Organizer: 第54回日本植物生理学会
  • Place of Presentation: 岩手大学（盛岡市）
• [Presentation] 全ゲノム重複後の重複遺伝子対の発現変化 -ポプラ・セルロース合成酵素の機能分化-. (2013)
  • Author(s): 高田直樹, 谷口亨
  • Organizer: 第63回日本木材学会
  • Place of Presentation: 岡山大学（岡山市）
• [Presentation] Functional divergence of secondary cell wall-associated CELLULOSE SYNTHASEs (CesAs) after a polyploidy event in Populus species (2012)
  • Author(s): Takata N, Taniguchi T
  • Organizer: Plant Biology Congress Freiburg 2012
  • Place of Presentation: Freiburg University (Freiburg, Germany)
  • Year and Date: 2012-07-31
• [Presentation] 二次細胞壁形成を担うポプラ・セルロース合成酵素の遺伝子発現制御と進化 (2012)
  • Author(s): 高田直樹, 谷口亨
  • Organizer: 第53回日本植物生理学会
  • Place of Presentation: 京都産業大学(京都)
  • Year and Date: 2012-03-18
• [Presentation] Functional divergence of secondary cell wall-associated CELLULOSE SYNTHASEs (CesAs) after a polyploidy event in Populus species. (2012)
  • Author(s): Takata N, Taniguchi T
  • Organizer: Plant Biology Congress Freiburg 2012
  • Place of Presentation: Albert-Ludwigs-University (Freiburg, Germany)
• [Presentation] 二次細胞壁形成を担うポプラ・セルロース合成酵素の遺伝子発現制御と進化 (2011)
  • Author(s): 高田直樹, 谷口亨
  • Organizer: 日本植物生理学会
  • Place of Presentation: 京都産業大学(京都府)
  • Year and Date: 2011-03-18