Analysis of clock oscillation in cancer tissue for effective chronotherapy to hypoxic area.

• Project/Area Number: 23890002
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Environmental physiology (including Physical medicine and Nutritional physiology)
• Research Institution: Hokkaido University
• Principal Investigator: MASUBUCHI Satoru 北海道大学, 大学院・医学研究科, 特任准教授 (80362771)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 概日リズム / 癌 / 低酸素 / 生物時計 / イメージング

Research Abstract

To monitor cancer circadian rhythm, DBPpromoter-luciferase (DBP-luc) stably introduced colon cancer cell line was established. We found heat shock (43C 1h) did not affects rhythm phase of DBP-luc cell. Additionally, hypoxia responsive element (HRE)-GFP was introduced to DBP-luc cell to monitor hypoxia and circadian rhythm simultaneously. To make DBP-luc / HRE-GFP stably introduced cell line by single transfection, we designed and made new gene constructs. We established the 3D in vitro DBP-luc cell culture system which mimics hypoxia of cancer tissue.

Research Products

• [Journal Article] Heteroplasmy of mouse mtDNA isgenetically unstable and results in altered behavior andcognition (2012)
  • Author(s): Sharpley MS, Marciniak C, Eckel-Mahan K, McManus M, Crimi M, Waymire K, Lin CS, Masubuchi S,Friend N, Koike M, Chalkia D,MacGregor G, Sassone-Corsi P, Wallace DC
  • Journal Title: Cell Volume: 151 Pages: 333-43
  • Peer Reviewed
• [Journal Article] 哺乳類の行動リズムと時計遺伝子 (2012)
  • Author(s): 増渕悟
  • Journal Title: 時間生物学 Volume: 18 Pages: 15-21
  • Peer Reviewed
• [Journal Article] 本間さと中枢体内時計と末梢体内時計のクロストーク (2012)
  • Author(s): 増渕悟,鈴木陽子
  • Journal Title: 日本臨牀 Volume: 70 Pages: 1127-32
• [Journal Article] Heteroplasmy of mouse mtDNA is genetically unstable and results in altered behavior and cognition. (2012)
  • Author(s): Sharpley MS
  • Journal Title: Cell Volume: 151 Issue: 2 Pages: 333-43
  • DOI: 10.1016/j.cell.2012.09.004
  • Peer Reviewed
• [Journal Article] 中枢体内時計と末梢体内時計のクロストーク． (2012)
  • Author(s): 増渕悟
  • Journal Title: 日本臨牀 Volume: 70 Pages: 1127-32
• [Journal Article] 中枢体内時計と末梢体内時計のクロストーク (2012)
  • Author(s): 増渕悟, 鈴木陽子, 本間さと
  • Journal Title: 日本臨牀 Volume: (印刷中)
• [Presentation] 概日リズムの制御機構 (2013)
  • Author(s): 増渕悟
  • Organizer: 弘前大学大学院セミナー
  • Place of Presentation: 弘前大学大学院医学研究科
  • Year and Date: 2013-01-09
• [Presentation] 低酸素と生物時計 (2012)
  • Author(s): 増渕悟
  • Organizer: 第56回未来医療イノベーションセミナー
  • Place of Presentation: 北海道大学大学院医学研究科
  • Year and Date: 2012-04-11
• [Presentation] ヒト大腸癌細胞株HCT116の概日遺伝子発現モニタリング (2012)
  • Author(s): 増渕悟, 本間さと, 本間研一
  • Organizer: 日本生理学会
  • Place of Presentation: 信州大学(松本市)
  • Year and Date: 2012-03-31
• [Presentation] Monitoring of circadian gene expression in human colon cancer cell line (2012)
  • Author(s): Satoru Masubuchi
  • Organizer: HCT116:"The 10th International Symposium for Future Drug Discovery and Medical Care" Sapporo
• [Presentation] ヒト大腸癌細胞株HCT116の概日遺伝子発現モニタリング (2012)
  • Author(s): 増渕悟、本間さと、本間研一
  • Organizer: 第19回時間生物学会
  • Place of Presentation: 北海道大学学術交流会館札幌
• [Presentation] Monitoring of circadian gene expression in human colon cancer cell line (2012)
  • Author(s): 増渕悟、本間さと、本間研一
  • Organizer: HCT11689回日本生理学会
  • Place of Presentation: 長野県松本文化会館、松本市総合体育館、信州大学松本キャンパス松本
• [Presentation] Monitoring of circadian gene expression in human colon cancer cell line, HCT116 (2012)
  • Author(s): 増渕悟
  • Organizer: The 10th International Symposium for Future Drug Discovery and Medical Care
  • Place of Presentation: 北海道大学医学部フラテホール（札幌）
• [Presentation] 時計遺伝子と行動リズム (2011)
  • Author(s): 増渕悟
  • Organizer: 第18回病理生命科学セミナー
  • Place of Presentation: 弘前大学大学院医学研究科
  • Year and Date: 2011-11-11
• [Presentation] Protein phosphatase PHLPP1 controls the light-induced resetting of the circadian clock (2011)
  • Author(s): Masubuchi S
  • Organizer: International Symposium on Photonic Bioimaging and Satellite Symposium of Worldsleep 2011 on Human Circadian Clock-the 50th anniversary of temporal isolation study
  • Place of Presentation: 北海道大学(札幌市)
  • Year and Date: 2011-10-22
• [Presentation] Phosphatase in suprachiamatic nucleus, PHLPP1/SCOP, controls period change (2011)
  • Author(s): Masubuchi S
  • Organizer: THIRD WORLD CONGRESS OF CHRONOBIOLOGY
  • Place of Presentation: BUAP Cultural Complex(プエブラ市、メキシコ)(招待講演)
  • Year and Date: 2011-05-05
• [Presentation] やわらかい時計 (2011)
  • Author(s): 増渕悟
  • Organizer: 第98回ニューロサイエンス談話会
  • Place of Presentation: 北海道大学大学院医学研究科
  • Year and Date: 2011-01-18
• [Presentation] Protein phosphatase PHLPP1 controls the light-induced resetting of the circadian clock.:International Symposium on Photonic Bioimaging and Satellite Symposium of Worldsleep 2011 on Human Circadian Clock - (2011)
  • Author(s): Satoru Masubuchi
  • Organizer: the 50th anniversary of temporal isolation study-
  • Place of Presentation: 京王プラザホテル札幌Sapporo
• [Presentation] Phosphatase in suprachiamatic nucleus, PHLPP1/SCOP, controls period change after light-induced phase shift,'After-Effect of Phase-Shift': Symposium 1 (2011)
  • Author(s): Satoru Masubuchi
  • Organizer: Cultural Centre BUAP
  • Place of Presentation: Puebla, Mexico