| Project/Area Number |
23890013
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Asahikawa Medical College |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 生殖工学 / 生殖補助技術 / 遺伝学的診断 / 染色体 |
|---|
| Research Abstract |
To produce offspring from pre-analyzed sperm, sperm DNA was replicated using androgenic embryos. Sister blastomeres from androgenic 2-cell embryos were individually fused to MIT oocytes to analyze sperm chromosomes and to produce offspring with known male chromosome constitutions. Also, Calyculin A facilitated the procedure in chromosome analysis of blastomere. These results demonstrated that sperm chromosome screening was successfully established.
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