Searching for a ligand that activates Gyc76C, a receptor-type guanylate cyclase which mediates innate immunity

• Project/Area Number: 23890014
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Biological pharmacy
• Research Institution: Tohoku University
• Principal Investigator: KURAISHI Takayuki 東北大学, 大学院・薬学研究科, 助教 (90613167)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 自然免疫学 / 自然免疫 / ショウジョウバエ / cGMP / グアニル酸シクラーゼ

Research Abstract

We have revealed that Gyc76C, a receptor-type guanylate cyclase, mediates innate immunity. In this study, we sought to identify a ligand for Gyc76C.
We found that Drosophila larval extract has an activity that induces the expression of antimicrobial peptides in Drosophila cell line. The activity was purified to homogeneity and the amino acid sequence was determined, resulting in theidentification of a new protein that activates innate immune system in Drosophila. Further analysis should be done to validate that whether the identified protein would be a ligand for Gyc76C.

Research Products

• [Journal Article] ショウジョウバエ腸管での免疫応答と制御 (2012)
  • Author(s): 倉石貴透、倉田祥一朗
  • Journal Title: 化学と生物 Volume: 50巻 Pages: 435-440
  • NAID: 10030820097
• [Journal Article] Apoptosis-dependent Externalization andInvolvement in Apoptotic Cell Clearance ofDmCaBP1, an Endoplasmic Reticulum Protein of Drosophila (2012)
  • Author(s): Okada R, Nagaosa K, Kuraishi T, Nakayama H, Yamamoto N, Nakagawa Y, Dohmae N, Shiratsuchi A, Nakanishi Y.
  • Journal Title: J Biol Chem Volume: 287巻 Pages: 3138-3146
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/22158613
  • Peer Reviewed
• [Journal Article] Apoptosis-dependent externalization and involvement in apoptotic cell clearance of DmCaBP1, an endoplasmic reticulum protein of Drosophila (2012)
  • Author(s): Okada, R., Nagaosa, K., Kuraishi, T., Nakayama, H., Yamamoto, N., Nakagawa, Y., Dohmae, N., Shiratsuchi, A., Nakanishi, Y
  • Journal Title: J. Biol. Chem Volume: 287 Issue: 5 Pages: 3138-3146
  • DOI: 10.1074/jbc.m111.277921
  • NAID: 120003959460
  • Peer Reviewed
• [Journal Article] Genetic evidence for a protective role of the peritrophic matrix against intestinal bacterial infection in Drosophila melanogaster (2011)
  • Author(s): Kuraishi T, Binggeli O, Opota O, BuchonN, Lemaitre B.
  • Journal Title: Proc Natl Acad Sci USA Volume: 108巻 Pages: 15966-15971
  • URL: http://www.pnas.org/content/early/2011/09/02/1105994108.abstract
  • Peer Reviewed
• [Journal Article] Mercury is a direct and potent γ-secretase inhibitor affecting Notch processing and development in Drosophila (2011)
  • Author(s): Alattia JR, Kuraishi T, Dimitrov M, Chang I, Lemaitre B, Fraering PC
  • Journal Title: FASEB J Volume: 25巻 Pages: 2287-2295
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/21415381
  • Peer Reviewed
• [Journal Article] Mercury is a direct and potent γ-secretase inhibitor affecting Notch processing and development in Drosophila (2011)
  • Author(s): Alattia JR, Kuraishi T, Dimitrov M, Chang I, Lemaitre B, Fraering PC
  • Journal Title: FASEB J. Volume: 25 Issue: 7 Pages: 2287-2295
  • DOI: 10.1096/fj.10-174078
  • Peer Reviewed
• [Journal Article] Genetic evidence for a protective role of the peritrophic matrix against intestinal bacterial infection in Drosophila melanogaster (2011)
  • Author(s): Kuraishi T, Binggeli O, Opota O, Buchon N, Lemaitre B
  • Journal Title: Proc Natl Acad Sci U S A Volume: 108 Issue: 38 Pages: 15966-15971
  • DOI: 10.1073/pnas.1105994108
  • Peer Reviewed
• [Presentation] ショウジョウバエ自然免疫におけるcGMP依存免疫応答活性化機構の解析 (2012)
  • Author(s): 狩野裕考、石川裕規、倉石貴透、倉田祥一朗
  • Organizer: 第78回日本生化学会東北支部会例会
  • Place of Presentation: 山形大学
  • Year and Date: 2012-05-26
• [Presentation] ショウジョウバエ受容体型グアニル酸シクラーゼによる血球細胞増殖を介した自然免疫制御機構の解明 (2012)
  • Author(s): 鈴木弘章、倉石貴透、倉田祥一朗
  • Organizer: 第85回生化学会大会
  • Place of Presentation: 福岡
• [Presentation] ショウジョウバエ受容体型グアニル酸シクラーゼによる血球細胞増殖を介した自然免疫制御機構の解明 (2012)
  • Author(s): 鈴木弘章、岩下真三、倉石貴透、後藤彰、倉田祥一朗
  • Organizer: 第23回日本生体防御学会学術総会
  • Place of Presentation: 東京
• [Presentation] 自然免疫応答を活性化するショウジョウバエ受容体型グアニル酸シクラーゼ下流のシグナル伝達分子の機能解析 (2012)
  • Author(s): 狩野裕考、石川裕規、倉石貴透、倉田祥一朗
  • Organizer: 日本薬学会第132年会
  • Place of Presentation: 札幌
• [Presentation] 自然免疫応答を活性化するショウジョウバエ受容体型グアニル酸シクラーゼ下流におけるシグナル伝達分子の同定 (2011)
  • Author(s): 狩野裕考、石川裕規、後藤彰、倉石貴透、倉田祥一朗
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-16
• [Presentation] 自然免疫応答を活性化するショウジョウバエ受容体型グアニル酸シクラーゼ下流におけるシグナル伝達分子の同定 (2011)
  • Author(s): 狩野裕考、石川裕規、後藤彰、倉石貴透、倉田祥一朗
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-16
• [Presentation] Genetic evidence for a protective role of the peritrophic matrix against intestinal bacterial infection in Drosophila melanogaster (2011)
  • Author(s): 倉石貴透, Olivier Binggeli, Onya Opota, Nicolas Buchon, Bruno Lemaitre
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-14
• [Presentation] ショウジョウバエ腸管免疫における囲食膜の役割 (2011)
  • Author(s): 倉石貴透, Olivier Binggeli, Onya Opota, Nicolas Buchon, Bruno Lemaitre
  • Organizer: 昆虫ワークショップ2011東北
  • Place of Presentation: 山形
  • Year and Date: 2011-10-14
• [Presentation] ショウジョウバエ自然免疫応答を活性化する受容体型グアニル酸シクラーゼ下流のシグナル伝達分子の同定 (2011)
  • Author(s): 狩野裕考、石川裕規、倉石貴透、倉田祥一朗
  • Organizer: 第10回次世代を担う若手ファーマ・バイオフォーラム2011
  • Place of Presentation: 仙台
  • Year and Date: 2011-10-09
• [Presentation] ショウジョウバエDnaJ様コシャペロン遺伝子CG8863の機能低下に伴う自然免疫の活性化 (2011)
  • Author(s): 熊田幸平、高柿武志、後藤彰、倉石貴透、大島吉輝、倉田祥一朗
  • Organizer: 第84回生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-23
• [Presentation] (2011)
  • Author(s): 倉石貴透, Olivier Binggeli, Onya Opota, Nicolas Buchon, Bruno LemaitreGenetic evidence for a protective role of the peritrophic matrix against intestinal bacterial infection in Drosophila melanogaster
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
• [Presentation] ショウジョウバエ腸管免疫における囲食膜 の役割 (2011)
  • Author(s): 倉石貴透,Olivier Binggeli,Onya Opota,Nicolas Buchon,Bruno Lemaitre
  • Organizer: 昆虫ワークショップ 2011東北
  • Place of Presentation: ZAOセンタープラザ 山形県山形市蔵王温泉903-2
• [Presentation] ショウジョウバエ自然免疫におけるcGMP依存免疫応答活性化機構の解析
  • Author(s): 狩野裕考、石川裕規、倉石貴透、倉田祥一朗
  • Organizer: 第78回日本生化学会東北支部会例会
  • Place of Presentation: 山形
• [Presentation] Drosophila immune response mediated by a receptor-type guanylate cyclase and a cyclic GMP-dependent protein kinase
  • Author(s): Hirotaka Kanoh, Takayuki Kuraishi, Hiroki Ishikawa, Shoichiro Kurata
  • Organizer: lnternational Symposium: Frotiers in Immunology and Inflammation
  • Place of Presentation: Tokyo
• [Remarks]
  • URL: http://www.pharm.tohoku.ac.jp/~seimei/seimei_original.html
• [Remarks] 東北大学大学院薬学研究科生命機能解析学分野
  • URL: http://www.pharm.tohoku.ac.jp/~seimei/seimei_original.html
• [Remarks]
  • URL: http://labtestsite.web.fc2.com/