In vivo analysis of novel type of adult human pluripotent stem cells.

• Project/Area Number: 23890015
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Tohoku University
• Principal Investigator: KURODA Yasumasa 東北大学, 大学院・医学系研究科, 助教 (00614504)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 多能性幹細胞 / 体性幹細胞 / 再生医療 / Muse細胞 / 細胞移植治療

Research Abstract

In this study, We tried to identify gene markers for isolating novel type of adult human pluripotent stem cells, Muse cells. Finally we were able to get some candidate from results of the next generation sequencing and the microarray analysis.

Research Products

• [Journal Article] Autologous mesenchymal stem cell-derived dopaminergic neurons function in parkinsonian macaques (2013)
  • Author(s): Hayashi T, Wakao S, Kitada M, Ose T, Watabe H, Kuroda Y, Mitsunaga K, Matsuse D, Shigemoto T, Ito A, Ikeda H, Fukuyama H, Onoe H, Tabata Y, Dezawa M
  • Journal Title: J Clin Invest Volume: 123(1) Issue: 1 Pages: 272-284
  • DOI: 10.1172/jci62516
  • Peer Reviewed
• [Journal Article] Transplantation of bone marrow stromal cell-derived neural precursor cells ameliorates deficits in a rat model of complete spinal cord transection (2013)
  • Author(s): Aizawa-Kohama M, Endo T, Kitada M, Wakao S, Sumiyoshi A, Matsuse D, Kuroda Y, Morita T, Riera JJ, Kawashima R, Tominaga T, Dezawa M
  • Journal Title: Cell Transplant Volume: 22(9) Issue: 9 Pages: 1613-25
  • DOI: 10.3727/096368912x658791
  • Peer Reviewed
• [Journal Article] Morphologic and gene expression criteria for identifying human induced pluripotent stem cells (2012)
  • Author(s): Wakao S, Kitada M, Kuroda Y, Ogura F, Murakami T, Niwa A, Dezawa M.
  • Journal Title: PLoS One Volume: 7 Issue: 12 Pages: e48677-e48677
  • DOI: 10.1371/journal.pone.0048677
  • Peer Reviewed
• [Journal Article] Isolation of adult human pluripotent stem cells from mesenchymal cell populations and their application to liver damages (2012)
  • Author(s): Wakao S, Kitada M, Kuroda Y, Dezawa M
  • Journal Title: Liver stem Cells : Methods and Protocols, Methods in Molecular Biology Volume: 826(2) Pages: 89-102
  • DOI: 10.1007/978-1-61779-468-1_8
  • ISBN: 9781617794674, 9781617794681
  • Peer Reviewed
• [Journal Article] Multiliineage-differentiating stress-enduring (Muse) cells are a primary source of induced pluripotent stem cells in human fibroblasts (2011)
  • Author(s): Wakao S, Kitada M, Kuroda Y, Shigemoto T, Matsuse D, Akashi H, Tanimura Y, Tsuchiyama K, Kikuchi T, Goda M, Nakahata T, Fijiyoshi Y, Dezawa M
  • Journal Title: Proc.Natl.Acad.Sci.U.S.A. Volume: 108(24) Issue: 24 Pages: 9875-9880
  • DOI: 10.1073/pnas.1100816108
  • Peer Reviewed
• [Journal Article] Lectins as a tool for detecting neural stem/progenitor cells in the adult mouse brain (2011)
  • Author(s): Kitada M, Kuroda Y, Dezawa M
  • Journal Title: Anat.Rec.(Hoboken) Volume: 294(2) Issue: 2 Pages: 305-321
  • DOI: 10.1002/ar.21311
  • Peer Reviewed
• [Journal Article] Mesenchymal stem cells and umbilical cord as sources for Schwann cell differentiation: their potential in peripheral nerve repair (2011)
  • Author(s): Kuroda Y, Kitada M, Wakao S, Dezawa, M.
  • Journal Title: The Open Tissue Engineering and Regenerative Medicine Journal Volume: 4 Issue: 1 Pages: 54-63
  • DOI: 10.2174/1875043501104010054
  • Peer Reviewed
• [Journal Article] Bone Marrow Mesenchymal Cells : How Do They Contribute to Tissue Repair and Are They Really Stem Cells? (2011)
  • Author(s): Kuroda Y, Kitada M, Wakao S, Dezawa M
  • Journal Title: Arch.Immunol.Ther.Exp.(Warsz) Volume: 59(5) Issue: 5 Pages: 369-378
  • DOI: 10.1007/s00005-011-0139-9
  • Peer Reviewed
• [Journal Article] 間葉系幹細胞における多様な分化と組織修復能を担うMuse細胞の発見 (2011)
  • Author(s): 黒田康勝、出澤真理
  • Journal Title: 血液フロンティア Volume: 21(11) Pages: 1664-1669
  • Peer Reviewed
• [Journal Article] 間葉系幹細胞の特性と再生医療における展開 (2011)
  • Author(s): 黒田康勝、出澤真理
  • Journal Title: 再生医療 Volume: 10(1) Pages: 8-11
  • Peer Reviewed
• [Presentation] 他家移植へ向けたMuse細胞の可能性 (2013)
  • Author(s): 黒田康勝、繁本妙子、出澤真理
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: 香川
• [Presentation] 他家移植へ向けた Muse 細胞の可能性 (2013)
  • Author(s): 黒田康勝
  • Organizer: 第118回日本解剖学会総会・全国学術集会
  • Place of Presentation: 香川
• [Presentation] 成人ヒト間葉系細胞に内在する新規多能性幹細胞の探索 (2012)
  • Author(s): 黒田康勝
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨大学
  • Year and Date: 2012-03-27
• [Presentation] 他家移植へ向けたMuse細胞の可能性 (2012)
  • Author(s): 黒田康勝、繁本妙子、出澤真理
  • Organizer: 解剖学会第58回東北・北海道連合支部学術集会
  • Place of Presentation: 山形
• [Presentation] 成人ヒト間葉系細胞に内在する多分化能細胞の探索 (2012)
  • Author(s): 黒田康勝, (他9名)
  • Organizer: 第11回日本再生医療学会総会
  • Place of Presentation: 横浜
• [Presentation] Novel type of adult human pluripotent stem cells that exist in mesenchymal cell population (2012)
  • Author(s): Yasumasa Kuroda, (他9名)
  • Organizer: The 5th Pan Pacific Symposium on Stem Cells and (他9名)Cancer Research
  • Place of Presentation: 台湾 (中華人民共和国)
• [Presentation] Novel type of adult human pluripotent stem cells that exist in mesenchymal cell population. (2012)
  • Author(s): Yasumasa Kuroda
  • Organizer: The 5th Pan Pacific Symposium on Stem Cells and Cancer Research
  • Place of Presentation: 台湾 (中華人民共和国)
• [Presentation] 成人ヒト間葉系細胞に内在する多分化能細胞の探索 (2012)
  • Author(s): 黒田康勝
  • Organizer: 第 11 回日本再生医療学会 総会
  • Place of Presentation: 横浜
• [Presentation] 他家移植へ向けた Muse 細胞の可能性 (2012)
  • Author(s): 黒田康勝
  • Organizer: 解剖学会第 58 回東北・北海道連合支部学術集会
  • Place of Presentation: 山形
• [Presentation] 成人ヒト間葉系細胞に内在する新規多能性幹細胞の探索 (2011)
  • Author(s): 黒田康勝
  • Organizer: 第57回日本解剖学会東北・北海道連合支部学術集会
  • Place of Presentation: 岩手大学
  • Year and Date: 2011-09-10
• [Remarks] 東北大学大学院医学系研究科細胞組織学分野・人体構造学分野
  • URL: http://www.stemcells.med.tohoku.ac.jp/index.html
• [Remarks] 東北大学大学院医学系研究科 細胞組織学分野・人体構造学分野
  • URL: http://www.stemcells.med.tohoku.ac.jp/index.html
• [Remarks]
  • URL: http://www.stemcells.med.tohoku.ac.jp/index.html