| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
Previously we identified the lung specific tissue stem cells named alveolar epithelial progenitor cells (AEPCs). The novel marine macrolide (-)-exiguolide significantly inhibit the proliferation of AEPCs. We hypothesized that (-)-exiguolide inhibit proliferation of lung cancer stem cells. Global gene expression analysis suggested the involvement of protein kinase C (PKC), extracellular regulated kinase and phosphoinositide 3 kinase (PI3K)/Akt signalling pathway in the tumour inhibition of (-)-exiguolide. Western blotting analysis confirmed the potential activity of (-)-exiguolide; p21 up-regulation through PKCα activation, MEK inhibition and PI3K/Akt/GSK3β pathway modulation resulting in apoptosis.
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