Induction of ameloblasts in high efficiency from induced pluripotent stem cells

• Project/Area Number: 23890022
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Tohoku University
• Principal Investigator: ARAKAKI Makiko 東北大学, 大学院・歯学研究科, 助教 (80610675)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: iPS細胞 / エナメル芽細胞 / 歯 / 再生 / 歯原性上皮細胞 / 人工多能性幹細胞（iPS細胞） / アメロブラスチン / 歯の再生 / 人工多能性幹細胞(iPS細胞)

Research Abstract

iPS cells are capable to be induced to ameloblasts when iPS cells were cultured with ameloblastin (Ambn) high expression dental epithelial cell line SF2-24, or cultured in the presence of conditioned medium (CM) of SF2-24 culture supernatant. Additionally, it is indicated that soluble factors such as Ambn, NT-4 and BMP, secreted from SF2 cells to culture medium, perform an important role for differentiation iPS cellsinto ameloblasts.

Research Products

• [Journal Article] Role of epithelial-stem cell interactions during dental cell differentiation (2012)
  • Author(s): Arakaki M, Ishikawa M, Nakamura T, Iwamoto T, Yamada A, Fukumoto E, Saito M, Otsu K, Harada H, Yamada Y, Fukumoto S
  • Journal Title: J Biol Chem Volume: 287(13) Issue: 13 Pages: 10590-601
  • DOI: 10.1074/jbc.m111.285874
  • Peer Reviewed
• [Journal Article] Role of epithelial-stem cell interactions during dental cell differentiation (2012)
  • Author(s): Arakaki M, Ishikawa M, Nakamura T, Iwamoto T, Yamada A, Fukuraoto E, Saito M, Otsu K, Harada H, Yamada Y, Fukumoto S
  • Journal Title: The Journal of Biological Chemistry Volume: 187(13) Pages: 10590-601
  • Peer Reviewed
• [Journal Article] Epithelial-mesenchymal interaction reduces inhibitory effects of fluoride on proliferation and enamel matrix expression in dental epithelial cells (2012)
  • Author(s): Yamada A, Iwamoto T, Fukumoto E, Arakaki M, Miyamoto R, Sugawara Y, Komatsu H, Nakamura T, Fukumoto S
  • Journal Title: Pediatric Dental Journal Volume: (未定)(in press)
  • NAID: 10030558189
  • Peer Reviewed
• [Journal Article] Epithelial cell lines in the field of dental research : review (2012)
  • Author(s): Fukumoto S, Arakaki M, Iwamoto T, Yamada A, Nakamura T
  • Journal Title: Interface Oral health Science Volume: (未定)(in press)
  • Peer Reviewed
• [Presentation] Dental cell differentiation from iPS cells (2013)
  • Author(s): Arakaki M.
  • Organizer: Sydney-Tohoku Symposium on Dentistry 2013
  • Place of Presentation: Sydney university, Australia
  • Year and Date: 2013-01-18
• [Presentation] iPS細胞からエナメル芽細胞への分化誘導技術の開発と歯の再生への応用 (2012)
  • Author(s): 新垣真紀子
  • Organizer: 第54回歯科基礎医学会学術大会
  • Place of Presentation: 奥羽大学
  • Year and Date: 2012-09-14
• [Presentation] Induction of ameloblasts from induced pluripotent stem cells (2012)
  • Author(s): Arakaki M, Nakamura T, Fukumoto S, et.al
  • Organizer: The 44th Annual Meeting for the Japanese Society of Developmental Biologists
  • Place of Presentation: 沖縄
  • Year and Date: 2012-05-20
• [Presentation] iPS細胞からエナメル芽細胞への分化誘導技術の開発と歯の再生への応用
  • Author(s): 新垣 真紀子
  • Organizer: 第54回歯科基礎医学会
  • Place of Presentation: 奥羽大学
• [Presentation] Dental cell differentiation from iPS cells
  • Author(s): Makiko Arakaki
  • Organizer: Sydney-Tohoku Symposium on Dentistry 2013
  • Place of Presentation: Sydney Dental Hospital
• [Presentation] Induction of ameloblasts from induced pluripotent stem (iPS) cells
  • Author(s): Makiko Arakaki
  • Organizer: 11th International Conference on Tooth Morphogenesis and differentiation
  • Place of Presentation: France Lyon
• [Remarks]
  • URL: http://www.tohoku.ac.jp/japanese/newimg/pressimg/tohokuuniv-press20120210_03.pdf