Effect of blastomere removal in the pre-implantation diagnosis on the epigenetic process and embryonic development
Project/Area Number |
23890023
|
Research Category |
Grant-in-Aid for Research Activity Start-up
|
Allocation Type | Single-year Grants |
Research Field |
Obstetrics and gynecology
|
Research Institution | Akita University |
Principal Investigator |
SATO Megumi 秋田大学, 医学部, 助教 (90609059)
|
Project Period (FY) |
2011-08-24 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥3,737,294 (Direct Cost: ¥2,874,842、Indirect Cost: ¥862,452)
Fiscal Year 2013: ¥487,294 (Direct Cost: ¥374,842、Indirect Cost: ¥112,452)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 着床前診断 / 割球細胞 / 胚発育 / 割球除去 / 胚発育挙動 |
Research Abstract |
The purpose of this study is to clarify how it affects the distribution of the remained blastomeres to eliminate one blastomere from embryo in embryonic development process. I examined the motion of one blastomere marked with fluorescent beads of the early mouse embryo in embryonic development. We also cultured and observed two-cell stage embryos which injected with beads to one blastomere. It was observed in the almost equal incidence rate that those beads are localized only in the inner cell mass or only in the cytotrophoblast cell layer. This study showed that there is a possibility that the fate of differentiation is determined to blastomeres that are considered to have a totipotency before embryonic genome transcription. This result indicates that we need additional investigation to confirm the safety of blastomere removal in the early embryo.
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Report
(4 results)
Research Products
(2 results)