Analysis of intracellular granule that recognizes viral infection

• Project/Area Number: 23890029
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: Chiba University
• Principal Investigator: ONOMOTO Koji 千葉大学, 真菌医学研究センター, 助教 (10612202)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 自然免疫 / RLR / インターフェロン / ウイルス / RNA / IFN / SG

Research Abstract

Emergence of new epidemic viruses such as swine flu and SARS spread rapidly and sometimes causes devastating impact. Innate immune system detects viral infection and protects host with viral sensors. Cytoplasmic viral RNA sensors, RLRs, sense viral RNA and initiate antiviral response, however it has been unclear how RLRs encounter viral RNA in cytoplasm. We revealed that RLRs localized in virus-induced granules together with viral RNA and trigger antiviral signal. We termed these aggregates as antiviral stress granules (avSGs).

Research Products

• [Journal Article] Functional characterization of domains of IPS-1 using an inducible oligomerization system (2013)
  • Author(s): Takamatsu S,Onoguchi K, Onomoto K, Narita R, Takahasi K, Ishidate F, Fujiwara TK, Yoneyama M, Kato H, Fujita T
  • Journal Title: PLoS One Volume: 8 Issue: 1 Pages: 1-9
  • DOI: 10.1371/journal.pone.0053578
  • NAID: 120005241348
  • Peer Reviewed
• [Journal Article] Foreign RNA induces the degradation of mitochondrial antiviral signaling protein (MAVS)the role of intracellular antiviral factors. (2012)
  • Author(s): Xing F, Matsumiya T, Onomoto K, Hayakari R, Imaizumi T, Yoshida H, Yoneyama M, Fijita T, Satoh K
  • Journal Title: PLoS One Volume: 7(9) Issue: 9 Pages: e45136-e45136
  • DOI: 10.1371/journal.pone.0045136
  • Peer Reviewed
• [Journal Article] Critical Role of an Antiviral Stress Granule Containing RIG-I and PKR in Viral Detection and Innate Immunity (2012)
  • Author(s): Onomoto K, Jogi M, Yoo J-S, Narita R, Morimoto S, Takemura A, Sambhara S, Kawaguchi A, Osari S, Nagata K, Matsumiya T, Namiki H, Yoneyama M, Fujita T
  • Journal Title: PLoS One Volume: 7 Issue: 8 Pages: 1-15
  • DOI: 10.1371/journal.pone.0043031
  • Peer Reviewed
• [Journal Article] Identification of germicidal compound againstpicornavirus in bamboo pyroligneous acid (2012)
  • Author(s): Marumoto S, Yamamoto SP, Nishimura H, Onomoto K, Yatagai M, Yazaki K, Fujita T, Watanabe T
  • Journal Title: Journal of Agricultural and Food chemistry Volume: 60(36) Issue: 36 Pages: 9106-9111
  • DOI: 10.1021/jf3021317
  • Peer Reviewed
• [Journal Article] Retinoic Acid-inducible Gene I-inducible miR-23b Inhibits Infections by Minor Group Rhinoviruses through Down-regulation of the Very Low Density Lipoprotein Receptor (2011)
  • Author(s): Ouda R, Onomoto K, Takahasi K, Edwards MR, Kato H, Yoneyama M, Fujita T
  • Journal Title: Journal of Biological Chemistry Volume: 286 Issue: 29 Pages: 26210-26219
  • DOI: 10.1074/jbc.m111.229856
  • Peer Reviewed
• [Journal Article] Dysregulation of IFN system can lead to poor response to pegylated interferon and ribavirin therapy in chronic hepatitis C (2011)
  • Author(s): Onomoto K, Morimoto S, Kawaguchi T, Toyoda H, Tanaka M, Kuroda M, Uno K, Kumada T, Matsuda F, Shimotohno K, Fujita T, Murakami Y
  • Journal Title: PLoS ONE Volume: 6 Issue: 5 Pages: e19799-e19799
  • DOI: 10.1371/journal.pone.0019799
  • Peer Reviewed
• [Presentation] Stress granule-like aggregate plays an important role for detecting of viral RNA by RIG-I-like receptors (2013)
  • Author(s): Onomoto K, Yoneyama M
  • Organizer: The 2nd Meeting on RNA and Biofunctions-ASIA Study “RNA biofunctions and Viruses”
  • Place of Presentation: ホテルレオパレス博多,(福岡)
• [Presentation] Analysis of Intracellular Localization of Viral RNA Sensor, RLR (2012)
  • Author(s): Onomoto K, Fujita T, Yoneyama M
  • Organizer: The 22nd CDB Meeting “RNA Sciences in Cell and Development Biology II”
  • Place of Presentation: RIKEN CDB, 神戸
• [Presentation] Subcellular localization of RIG-I-like receptors in virus-infected cells (2011)
  • Author(s): Yoneyama M, Onomoto K, Jogi M, Fujita T
  • Organizer: 9^<th> Joint Meeting of International Cytokine Society and International Society for Interferon and Cytokine Research
  • Place of Presentation: Florence, Italy
  • Year and Date: 2011-10-10
• [Presentation] Granular-like accumulation of RIG-I-like receptors in virus-infected cells (2011)
  • Author(s): Jogi M, Onomoto K, Fujita T, Yoneyama M
  • Organizer: XV International Congress of Virology
  • Place of Presentation: 札幌コンベンションセンター,札幌
  • Year and Date: 2011-09-15
• [Presentation] Detection of viral non-self RNA by RIG-I-like receptors in cytoplasmic granule-like structure (2011)
  • Author(s): Yoneyama M, Onomoto K, Jogi M, Fujita T
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道大学,札幌(招待講演)
  • Year and Date: 2011-06-29
• [Presentation] Analysis of intracellular localization of viral RNA sensor (2011)
  • Author(s): Onomoto K, Yoneyama M, Fujita T
  • Organizer: Experimental Biology 2011 (Joint Meeting of AAA, AOS, ASBMB, ASIP, ASN and ASPET)
  • Place of Presentation: Washington DC, USA
  • Year and Date: 2011-04-13
• [Book] 実験医学 増刊 感染・共生・生体防御システム ウイルスセンサーによるRNA認識とシグナル活性化の分子メカニズム (2012)
  • Author(s): 尾野本浩司、米山光俊
  • Total Pages: 8
  • Publisher: 羊土社
• [Book] 米山光俊ウイルスセンサーによるRNA認識とシグナル活性化の分子メカニズム実験医学増刊号「感染・共生・生体防御システム」
  • Author(s): 尾野本浩司
  • Publisher: 羊土社
• [Remarks] 葉大学真菌医学研究センター
  • URL: http://myco2.pf.chiba-u.jp/index.html
• [Remarks] 千葉大学真菌医学研究センタープロフィールページ
  • URL: http://myco2.pf.chiba-u.jp/profile/profile_onomoto.html
• [Remarks] 千葉大学真菌医学研究センター感染免疫分野ホームページ
  • URL: http://myco2.pf.chiba-u.jp/bunya_kansenmeneki/bunya_kansenmeneki/Mol._Immunol._jpn.html
• [Remarks] 千葉大学真菌医学研究センター 感染免疫分野
  • URL: http://www.pf.chiba-u.ac.jp/bunya_kansenmeneki.html
• [Remarks] 千葉大学真菌医学研究センター 感染免疫分野 尾野本プロファイル
  • URL: http://www.pf.chiba-u.ac.jp/profile/profile_onomoto.html
• [Remarks] 千葉大学真菌医学研究センター 感染免疫分野 ホームページ
  • URL: http://www.pf.chiba-u.ac.jp/bunya_kansenmeneki/bunya_kansenmeneki/Mol._Immunol._jpn.html
• [Remarks]
  • URL: http://www.pf.chiba-u.ac.jp/bunya_kansenmeneki.html
• [Remarks]
  • URL: http://www.pf.chiba-u.ac.jp/bunya_kansenmeneki/bunya_kansenmeneki/Mol._Immunol._jpn.html