Role of CD69 for the generation of memory T helper lymphocytes

• Project/Area Number: 23890030
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Immunology
• Research Institution: Chiba University
• Principal Investigator: SHINODA Kenta 千葉大学, 大学院・医学研究院, 特任助教 (10612195)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: アレルギー・喘息 / 免疫学 / アレルギー･喘息

Research Abstract

Memory T helper (Th) lymphocytes play an essential role in immunologicalmemory. However, little is known about the molecular mechanisms of the generation and maintenanceof memory Th cells. We focused on CD69 and examined to clarify the role of CD69 in memory Th cells.Although CD69 does not appear to be required for the development of effector Th cells, the number ofantigen-specific memory Th cells in CD69-deficient mice dramatically decreased compared to wild-typemice. CD69 plays a crucial role in the generation of memory Th cells and that the relocation of effectorTh cells to the BM is essential for generation of memory Th cells .

Research Products

• [Journal Article] Regulation of memory CD4 T-cell pool size and function by natural killer T cells in vivo. 109(42) : 16992-16997 (2012). (2012)
  • Author(s): Iwamura, C., Shinoda, K., Endo, Y., Watanabe, Y., Tumes, J. D., Motohashi, S., Kawahara, K., Kinjo, Y., and Nakayama, T.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 109 Issue: 42 Pages: 16992-16997
  • DOI: 10.1073/pnas.1203494109
  • Peer Reviewed
• [Journal Article] The transcription factor Sox4 is a downstream target of signaling by the cytokine TGF-・ and suppresses TH2 differentiation (2012)
  • Author(s): Kuwahara, M., Yamashita, M., Shinoda, K., Tofukuji, S., Onodera, A., Shinnakasu, R., Motohashi, S., Hosokawa, H., Tumes, D., Iwamura, C., Lefebvre, V., and Nakayama, T.
  • Journal Title: Nat. Immunol Volume: 13(8) Issue: 8 Pages: 778-786
  • DOI: 10.1038/ni.2362
  • Peer Reviewed
• [Journal Article] Type II membrane protein CD69 regulates the formation of resting T-helper memory (2012)
  • Author(s): Shinoda, K., Tokoyoda, K., Hanazawa, A., Hayashizaki, K., Zehentmeier, S., Hosokawa, H., Iwamura, C, Koseki, H., Tumes, D.J., Radbruch, A., Nakayama T.
  • Journal Title: Proc.Natl.Acad.Sci.USA Volume: (in press) Issue: 19 Pages: 7409-7414
  • DOI: 10.1073/pnas.1118539109
  • Peer Reviewed
• [Journal Article] Murine Schnurri-2 controls Natural Killer cell function and lymphoma development (2012)
  • Author(s): Yamashita, J., Iwamura, C., Mitsumori, K., Hosokawa, H., Sasaki, T., Takahashi, M., Tanaka, H., Kaneko, K., Hanazawa, A., Watanabe, Y., Shinoda, K., Tumes, D., Motohashi, S., Nakayama, T.
  • Journal Title: Leuk.Lymphoma Volume: 53 Issue: 3 Pages: 479-486
  • DOI: 10.3109/10428194.2011.625099
  • Peer Reviewed
• [Journal Article] ncrease of regulatory T cells and the ratio of specific IgE to total IgE are candidates for response monitoring or prognostic biomarkers in 2-year sublingual immunotherapy (SLIT) for Japanese cedar pollinosis (2011)
  • Author(s): Fujimura, T., Yonekura, S., Horiguchi, S., Taniguchi, Y., Saito, A., Yasueda, H., Inamine, A., Nakayama, T., Takemori, T., Taniguchi, M., Sakaguchi, M. Okamoto, Y
  • Journal Title: Clin. Immunol Volume: 139 Issue: 1 Pages: 65-74
  • DOI: 10.1016/j.clim.2010.12.022
  • Peer Reviewed
• [Presentation] Toshinori Nakayama Generationand Maintenance of memory T-helperlymphocytes (2013)
  • Author(s): Kenta Shinoda, Chiaki Iwamura, Koji Tokoyoda
  • Organizer: The 1^<st> Symposium ofInternational Immunological Memory and Vaccine Forum (IIMVF)
  • Year and Date: 2013-01-29
• [Presentation] CD69 regulates the formation of resting T helper memory (2012)
  • Author(s): Shinoda K., Nakayama T.
  • Organizer: 14th International Conference on Lymphocyte Activation and Immune Regulation
  • Place of Presentation: NewPort Beach, USA
  • Year and Date: 2012-02-05
• [Presentation] CD69 regulates the formation of resting T-helper memory (2012)
  • Author(s): Shinoda, K., Tokoyoda, K., Hanazawa, A., Hayashizaki, K., Hosokawa, H., Iwamura, C., Koseki, H., Tumes, D., Radbruch, A., andNakayama, T.
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 神戸
• [Presentation] CD69regulates the formation of resting T-helpermemory (2012)
  • Author(s): Shinoda, K., Tokoyoda, K., Hanazawa, A.,Hayashizaki, K., and Nakayama, T.
  • Organizer: THE 34th NAITO CONFERENCEON Infection, Immunity, and their control forHealth
  • Place of Presentation: Sapporo
• [Presentation] Generation and maintenance ofmemory CD4 T cells. (招待講演) (2012)
  • Author(s): Nakayama, T., Endo, Y., Kuwahara, M.,Yamashita, M., Iwamura, C., Shinoda, K., andTokoyoda, K.
  • Organizer: THE 34thNAITO CONFERENCE ON Infection,Immunity, and their control for Health
  • Place of Presentation: Sapporo
• [Presentation] CD69は記憶ヘルパーT細胞の形成を制御する (2012)
  • Author(s): 篠田健太、常世田好司、花澤麻美、林崎浩史、中山俊憲
  • Organizer: 第22回Kyoto T Cell Conference
  • Place of Presentation: 和順会館
• [Presentation] CD69 regulates theformation of resting T helper memory (2012)
  • Author(s): Shinoda K., Nakayama T
  • Organizer: 14th International Conference on Lymphocyte Activation and Immune Regulation
  • Place of Presentation: NewPort Beach, USA
• [Presentation] Generation and maintenance of memory CD4 T cells (2012)
  • Author(s): Nakayama, T., Endo, Y., Kuwahara, M., Yamashita, M., Iwamura, C., Shinoda, K., and Tokoyoda, K.
  • Organizer: The 34th NAITO CONFERENCE ON Infection, Immunity, and their control for Health
  • Place of Presentation: 札幌
  • Invited
• [Presentation] CD69 regulates the formation of resting T-helper memory (2012)
  • Author(s): Shinoda, K., Tokoyoda, K., Hanazawa, A., Hayashizaki, K., and Nakayama, T.
  • Organizer: The 34th NAITO CONFERENCE ON Infection, Immunity, and their control for Health
  • Place of Presentation: 札幌
• [Presentation] 骨髄におけるTヘルパー記憶の成立/Establishment of T helper memory in bone marrow (2011)
  • Author(s): Tokoyoda, K., Shinoda, K., Hanazawa, A., Hayashizaki, K., Radbruch, A., Nakayama, T.
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張
  • Year and Date: 2011-11-28
• [Presentation] 活性化NKT細胞によるメモリーTh2細胞の増加と機能変化/Selective expansion and functional modulation of memory Th2 cells by activated NKT cells in vivo (2011)
  • Author(s): 岩村千秋、篠田健太、高橋克己、中山俊憲
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張
  • Year and Date: 2011-11-28
• [Presentation] Sox4はTGFbにより誘導され、Th2型免疫反応を抑制する/Sox4 acts as a downstream target of TGFb and suppresses Th2 type immune responses (2011)
  • Author(s): Kuwahara, M., Iwamura, C., Shinoda, K., Tofukuji, S., Suzuki, J., Nakayama, T., Yamashita, M.
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張
  • Year and Date: 2011-11-27
• [Presentation] ROGはTh2による肥満細胞の脱顆粒を制御する事で接触性皮膚炎を抑制する/Repressor of GATA negatively regulates the induction of contact hypersensitivity via Th2-induced mast cell degranulation (2011)
  • Author(s): Hirasaki, Y., Iwamura, C., Yamashita, M., Ito, T., Shinoda, K., Nakayama, T
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張
  • Year and Date: 2011-11-27
• [Presentation] HMG box型転写因子Sox4はTGF-b刺激で誘導され、Th2細胞分化を抑制する (2011)
  • Author(s): 桑原誠、岩村千秋、篠田健太、東福寺聡一、鈴木淳平、中山俊憲、山下政克
  • Organizer: 第21回Kyoto T Cell Conference
  • Place of Presentation: 京都
  • Year and Date: 2011-06-11
• [Presentation] Selective expansion and functional modulation of memory Th2 cells by activated NKT cells in vivo (2011)
  • Author(s): 岩村千秋、篠田健太、花澤麻美、中山俊憲
  • Organizer: 第21回Kyoto T Cell Conference
  • Place of Presentation: 京都
  • Year and Date: 2011-06-11
• [Presentation] 活性化NKT細胞によるメモリーTh2 細胞の増加と機能変化/ Selective expansion andfunctional modulation of memory Th2 cells byactivated NKT cells in vivo (2011)
  • Author(s): 岩村千秋、篠田健太、高橋克己、中山俊憲
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張
• [Presentation] 骨髄における T ヘルパ-記憶の成立/Establishment of T helper memory in bonemarrow (2011)
  • Author(s): Tokoyoda, K., Shinoda, K., Hanazawa, A.,Hayashizaki, K., Radbruch, A., and Nakayama,T.
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張
• [Presentation] ROGはTh2による肥満細胞の脱顆粒を制御する事で接触性皮膚炎を抑制する/Repressor of GATA negatively regulates the induction of contacthypersensitivity via Th2-induced mast cell degranulation (2011)
  • Author(s): Hirasaki, Y., Iwamura, C., Yamashita, M., Ito, T.,Shinoda, K., and Nakayama, T.
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張
• [Presentation] Sox4 は TGFbにより誘導され、Th2型免疫反応を抑制する/Sox4 acts as a downstream target of TGFb and suppresses Th2 type immune responses (2011)
  • Author(s): Kuwahara, M., Iwamura, C., Shinoda, K.,Tofukuji, S., Suzuki, J., Nakayama, T., andYamashita, M.
  • Organizer: 第40回日本免疫学会学術集会
  • Place of Presentation: 幕張
• [Presentation] Selective expansion and functional modulation of memory Th2 cells by activated NKT cells in vivo (2011)
  • Author(s): 岩村千秋、篠田健太、花澤麻美、中山俊憲
  • Organizer: 第21回 Kyoto T Cell Conference
  • Place of Presentation: 京都
• [Presentation] HMGbox型転写因子Sox4はTGF-b刺激で誘導され、Th2細胞分化を抑制する (2011)
  • Author(s): 桑原誠、岩村千秋、篠田健太、東福寺聡一、鈴木淳平、中山俊憲、山下政克
  • Organizer: 第21回Kyoto T CellConference
  • Place of Presentation: 京都
• [Book] 記憶ヘルパーT細胞形成におけるCD69の役割 (2012)
  • Author(s): 篠田健太、中山俊憲
  • Publisher: 医学のあゆみ