Development of p53 expressing viral vector using human insulator.

Research Project

Project/Area Number	23890046
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Obstetrics and gynecology
Research Institution	The University of Tokyo
Principal Investigator	MORI Mayuyo 東京大学, 医学部付属病院, 登録研究医 (30570452)
Project Period (FY)	2011 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000) Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	p53・インスレーター / インスレーター / ベクター / p53
Research Abstract	Efficient and continuous expression of a therapeutic transgene is a key factor for improving the efficacy of gene therapy. Some insulators are known to contribute to continuous high-level expression of a therapeutic transgene. In this study, we modified an adeno-associated virus serotype 2 (AAV2) vector expressing p53 from the mouse muscle creatine kinase gene promoter-enhancer (Ckm).