| Project/Area Number |
23890070
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Orthopaedic surgery
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| Research Institution | Kanazawa University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
|
| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 骨肉腫 / 肺転移 / プラスミノーゲン活性化因子インヒビタ-1 (PAI-1) / ウロキナーゼ型プラスミノ-ゲン活性化因子 / 蛍光蛋白 / uPA / PAI-1 / 凝固・線溶因子 |
|---|
| Research Abstract |
With use of human osteosarcoma cell line “143B dual color”, in which GFP is expressing in nucleus and RFP is expressing in cytoplasm, we first developed multiple cell lines that have multiple metastatic potential. In each cell line, we evaluated expression level of uPA, PAI-1, and TF by ELIZA and PCR. The result showed strong relationship between metastatic potential and expression of uPA & PAI-1. We have now performed shRNA of uAP & PAI-1 for 143B cell line. Then we will evaluate how metastatic potential change after suppression of uPA and PAI-1 in osteosarcoma.
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