Molecular mechanism of epigenetic alteration associated with lung metastasis formation in colorectal cancer and therapeutic application based on the mechanism.

• Project/Area Number: 23890083
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Digestive surgery
• Research Institution: Mie University
• Principal Investigator: FUJIKAWA Hiroyuki 三重大学, 医学部附属病院, 医員 (40616091)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 大腸癌 / 遠隔転移 / microRNA / epigenetic / 臓器特異性転移 / メチル化 / micro RNA / 遺伝子発現

Research Abstract

We showed that EMT-MET switch are regulated by promoter methylation status of CpG island of miR-200c family and expression of miR-200c in metastatic sites is significantly higher than that in primary sites. In addition, we demonstrated that serum miR-200c has a strong potential to serve as a noninvasive biomarker for CRC prognosis and predicting metastasis.

Research Products

• [Journal Article] Serum miR-200c is a novel prognostic and metastasis-predictive biomarker in patients with colorectal cancer (2013)
  • Author(s): Toiyama Y, Hur K, Tanaka K, Inoue Y, Kusunoki M, Boland CR, Goel A
  • Journal Title: Annals of Surg Volume: (in press)
  • Peer Reviewed
• [Journal Article] Macrophage inflammatory protein-3 alpha (MIP-3a) is a novel serum prognostic marker in patients with colorectal cancer. (2013)
  • Author(s): Iwata T, Tanaka K, Inoue Y, Toiyama Y, Hiro J, Fujikawa H, Okugawa Y, Uchida K, Mohri Y, Kusunoki M
  • Journal Title: J Surg Oncol Volume: 107 Pages: 160-6
  • Peer Reviewed
• [Journal Article] Macrophage inflammatory protein-3 alpha (MIP-3a) is a novel serum prognostic marker in patients with colorectal cancer. (2013)
  • Author(s): Iwata T
  • Journal Title: J Surg Oncol. Volume: 107 Issue: 2 Pages: 160-166
  • DOI: 10.1002/jso.23247
  • Peer Reviewed
• [Journal Article] Serum miR-200c is a novel prognostic and metastasis-predictive biomarker in patients with colorectal cancer. (2013)
  • Author(s): Toiyama Y
  • Journal Title: Annals of Surg. Volume: in press
  • Peer Reviewed
• [Journal Article] MicroRNA-200c modulates epithelial-to-mesenchymal transition (EMT) in human colorectal cancer metastasis (2012)
  • Author(s): Hur K, Toiyama Y, Takahashi M, Balaguer F, Nagasaka T, Koike J, Hemmi H, Koi M, Boland CR, Goel A
  • Journal Title: Gut
  • Peer Reviewed
• [Journal Article] High TrkB expression levels are associated with poor prognosis and EMT induction in colorectal cancer cells (2012)
  • Author(s): Fujikawa H, Tanaka K, Toiyama Y, Saigusa S, Inoue Y, Uchida K, Kusunoki M
  • Journal Title: J Gastroenterol Volume: 47 Pages: 775-84
  • NAID: 10030962144
  • Peer Reviewed
• [Journal Article] Clinical significance of serum soluble E-cadherin in colorectal carcinoma (2012)
  • Author(s): Okugawa Y, Toiyama Y, Inoue Y, Iwata T, Fujikawa H, Saigusa S, Konishi N, Tanaka K, Uchida K, Kusunoki M
  • Journal Title: J Surg Res Volume: 175
  • Peer Reviewed
• [Journal Article] Prognostic significance of glucose transporter-1 (GLUT1) gene expression in rectal cancer after preoperative chemoradiotherapy (2012)
  • Author(s): Saigusa S, Toiyama Y, Tanaka K, Okugawa Y, Fujikawa H, Matsushita K, Uchida K, Inoue Y, Kusunoki M
  • Journal Title: Surg Today Volume: 42 Pages: 460-9
  • NAID: 10030621686
  • Peer Reviewed
• [Journal Article] Evaluation of CXCL10 as a novel serum marker for predicting liver metastasis and prognosis in colorectal cancer (2012)
  • Author(s): Toiyama Y, Fujikawa H, Kawamura M, Matsushita K, Saigusa S, Tanaka K, Inoue Y, Uchida K, Mohri Y, Kusunoki M
  • Journal Title: Int J Oncol Volume: 40 Pages: 560-6
  • Peer Reviewed
• [Journal Article] Inhibition of HGF/cMET expression prevents distant recurrence of rectal cancer after preoperative chemoradiotherapy (2012)
  • Author(s): Saigusa S, Toiyama Y, Tanaka K, Yokoe T, Fujikawa H, Matsushita K, Okugawa Y, Inoue Y, Uchida K, Mohri Y, Kusunoki M
  • Journal Title: nt J Oncol Volume: 40 Pages: 583-91
  • Peer Reviewed
• [Journal Article] Co-expression of hepatocyte growth factor and c-Met predicts peritoneal dissemination established by autocrine hepatocyte growth factor/c-Met signaling in gastric cancer (2012)
  • Author(s): Toiyama Y, Yasuda H, Saigusa S, Matushita K, Fujikawa H, Tanaka K, Mohri Y, Inoue Y, Goel A, Kusunoki M
  • Journal Title: Int J Cancer Volume: 130 Pages: 2912-21
  • Peer Reviewed
• [Journal Article] Co-expression of hepatocyte growth factor and c-Met predicts peritoneal dissemination established by autocrine hepatocyte growth factor/c-Met signaling in gastric cancer. (2012)
  • Author(s): Toiyama Y, Yasuda H, et al.
  • Journal Title: Int J Cancer Volume: 130(12) Issue: 12 Pages: 2912-21
  • DOI: 10.1002/ijc.26330
  • Peer Reviewed
• [Journal Article] Inhibition of HGF/cMET expression prevents distant recurrence of rectal cancer after preoperative chemoradiotherapy. (2012)
  • Author(s): Saigusa S
  • Journal Title: Int J Oncol. Volume: 40 Pages: 583-591
  • DOI: 10.3892/ijo.2011.1200
  • Peer Reviewed
• [Journal Article] Evaluation of CXCL10 as a novel serum marker for predicting liver metastasis and prognosis in colorectal cancer. (2012)
  • Author(s): Toiyama Y
  • Journal Title: Int J Oncol Volume: 40 Pages: 560-566
  • DOI: 10.3892/ijo.2011.1247
  • Peer Reviewed
• [Journal Article] Prognostic significance of glucose transporter-1 (GLUT1) gene expression in rectal cancer after preoperative chemoradiotherapy. (2012)
  • Author(s): Saigusa S
  • Journal Title: Surg Today Volume: 42 Issue: 5 Pages: 460-469
  • DOI: 10.1007/s00595-011-0027-2
  • Peer Reviewed
• [Journal Article] Clinical significance of serum soluble E-cadherin in colorectal carcinoma. (2012)
  • Author(s): Okugawa Y
  • Journal Title: J Surg Res Volume: 175 Issue: 2 Pages: e67-e73
  • DOI: 10.1016/j.jss.2011.11.009
  • Peer Reviewed
• [Journal Article] High TrkB expression levels are associated with poor prognosis and EMT induction in colorectal cancer cells. (2012)
  • Author(s): Fujikawa H, Tanaka K, et al
  • Journal Title: J Gastroenterol. Volume: 47 Issue: 7 Pages: 775-784
  • DOI: 10.1007/s00535-012-0532-0
  • NAID: 10030962144
  • Peer Reviewed
• [Journal Article] MicroRNA-200c modulates epithelial-to-mesenchymal transition (EMT) in human colorectal cancer metastasis. (2012)
  • Author(s): Hur K
  • Journal Title: Gut Volume: ahead of print
  • Peer Reviewed
• [Journal Article] Evaluation of CXCL10 as a novel serum marker for predicting liver me tastasis and prognosis in colorectal cancer (2012)
  • Author(s): Toiyama Y, Fujikawa H, et al
  • Journal Title: Int J Oncol Volume: 40(2) Pages: 560-6
  • Peer Reviewed
• [Journal Article] Inhibition of HGF/cMET expression prevents distant recurrence of rectal cancer after preoperative chemoradiotherapy (2012)
  • Author(s): Saigusa S, Fujikawa H, et al
  • Journal Title: Int J Oncol Volume: 40(2) Pages: 583-91
  • Peer Reviewed
• [Journal Article] Cancer-associated fibroblasts correlate with poor prognosis in rectal cancer after chemoradiotherapy (2011)
  • Author(s): Saigusa S, Toiyama Y, Tanaka K, Yokoe T, Okugawa Y, Fujikawa H, Matsusita K, Kawamura M, Inoue Y, Miki C, Kusunoki M
  • Journal Title: Int J Oncol Volume: 38 Pages: 655-63
  • Peer Reviewed
• [Journal Article] Cancer-associated fibroblasts correlate with poor prognosis in rectal cancer after chemoradiotherapy (2011)
  • Author(s): Saigusa S, Fujikawa H, et al
  • Journal Title: Int J Oncol Volume: 38(3) Pages: 655-63
  • Peer Reviewed
• [Presentation] 大腸癌における術前血清CXCL10発現の意義 (2011)
  • Author(s): 藤川裕之, ほか
  • Organizer: 日本外科学会
  • Place of Presentation: 東京(紙上開催に変更)