Anti-biofilm effects of azithromycin on Porphyromonas gingivalis

• Project/Area Number: 23890103
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Conservative dentistry
• Research Institution: Osaka University
• Principal Investigator: MAEZONO Hazuki 大阪大学, 歯学部附属病院, 医員 (00613390)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: バイオフィルム / 抗生物質 / ポルフィロモナス / ジンジバリス / 歯学 / ポルフィロモナス ジンジバリス / ポルフィロモナス ジンジバリス

Research Abstract

Anti-biofilm effects of azithromycin (AZM) on Porphyromonas gingivaliswere investigated using modified Robbins device. We examined protein expressions of AZM-treated/non-treated Porphyromonas gingivalis and found that protein expressions of sub-MIC AZM treated planktonic and biofilm-detached cells were different at 50 and 110 kDa. Proteins were turned out to be glutamate dehydrogenase, hemagglutinin protein and RagA protein.

Research Products

• [Journal Article] Porphyromonas gingivalis biofilms persist after chlorhexidine treatment (2013)
  • Author(s): Yamaguchi M, Noiri Y, Kuboniwa M, Yamamoto R, Asahi Y, Maezono H, Hayashi M, Ebisu S
  • Journal Title: Eur J Oral Sci Volume: Vol.121 Issue: 3pt1 Pages: 162-8
  • DOI: 10.1111/eos.12050
  • Peer Reviewed
• [Journal Article] The sinR ortholog PGN_0088 encodes a transcriptional regulator that inhibits polysaccharide synthesis in Porphyromonas gingivalis ATCC 33277 biofilms (2013)
  • Author(s): Yamamoto R, Noiri Y, Yamaguchi M, Asahi Y, Maezono H, Kuboniwa M, Hayashi M, Ebisu S
  • Journal Title: PLoS One Volume: Vol.8, No.2 Issue: 2 Pages: e56017-e56017
  • DOI: 10.1371/journal.pone.0056017
  • Peer Reviewed
• [Journal Article] Anti-biofilm effects of azithromycin and erythromycin on Porphyromonas gingivalis (2011)
  • Author(s): Maezono H, Noiri Y, Asahi Y, Yamaguchi M, Yamamoto R, Izutani N, Azakami H, Ebisu S
  • Journal Title: Antimicrob Agents Chemother Volume: 55 Issue: 12 Pages: 5887-5892
  • DOI: 10.1128/aac.05169-11
  • Peer Reviewed
• [Journal Article] Time-course gene expression during Porphyromonas gingivalis strain ATCC 33277 biofilm formation (2011)
  • Author(s): Yamamoto R, Noiri Y, Yamaguchi M, Asahi Y, Maezono H, Ebisu S
  • Journal Title: Appl Environ Microbiol Volume: 77 Issue: 18 Pages: 6733-6736
  • DOI: 10.1128/aem.00746-11
  • Peer Reviewed
• [Presentation] Azithromycin alters protein expressions in Porphyromonas gingivalisbiofilm-detached cells (2012)
  • Author(s): H. MAEZONO, Y. NOIRI, Y. ASAHI, M. YAMAGUCHI, R. YAMAMOTO, M. HAYASHI and S. EBISU
  • Organizer: 第60回国際歯科研究学会日本部会(JADR)
  • Place of Presentation: 新潟県新潟市
  • Year and Date: 2012-12-15
• [Presentation] Altered protein expressions by sub-minimum inhibitory concentrations of azithromycin on Porphyromonas gingivalis biofilms (2012)
  • Author(s): H. MAEZONO, Y. NOIRI, Y. ASAHI, M. YAMAGUCHI, R. YAMAMOTO, K. KUREMOTO, M. HAYASHI and S. EBISU
  • Organizer: ASM 6thconference on biofilms
  • Place of Presentation: Miami, USA
  • Year and Date: 2012-10-03
• [Presentation] Porpbyromonas gingivalis PGN_0088遺伝子のバイオフィルム形成における役割 (2012)
  • Author(s): 山本れいこ, ら
  • Organizer: 第85回日本細菌学会総会
  • Place of Presentation: 長崎ブリックホール(長崎)
  • Year and Date: 2012-03-29
• [Presentation] Sub-MICの緑茶カテキンがPorpbyromonas gingivalisバイオフィルムに及ぼす影響の検索 (2011)
  • Author(s): 朝日陽子, ら
  • Organizer: 第135回日本歯科保存学会秋季学術大会
  • Place of Presentation: 大阪国際交流センター(大阪)
  • Year and Date: 2011-10-20
• [Presentation] Remnant matrix of Porphyromonas gingivalis biofilm treated with chlorhexidine (2011)
  • Author(s): Yamaguchi M, et al
  • Organizer: International Union of Microbial Societies 2011 Congress
  • Place of Presentation: Sapporo (Japan)
  • Year and Date: 2011-09-08
• [Presentation] Porphyromonas gingivalisのバイオフィルムに対するクロルヘキシジンの影響 (2011)
  • Author(s): 山口幹代, ら
  • Organizer: 第25回Bacterial Adherence & Biofilm
  • Place of Presentation: 京王プラザホテル(東京)
  • Year and Date: 2011-07-08
• [Presentation] Porphyromonas gingivalisバイオフィルムに対する各種抗菌薬の抗バイオフィルム効果 (2011)
  • Author(s): 前薗葉月、野杁由一郎、朝日陽子、山口幹代、山本れいこ、薮根敏晃、恵比須繁之
  • Organizer: 第134回日本歯科保存学会
  • Place of Presentation: 千葉県浦安市
  • Year and Date: 2011-06-10
• [Presentation] Porphyromonas gingivalisバイオフィルムに対する各種抗菌薬の抗バイオフィルム効果 (2011)
  • Author(s): 前薗葉月, ら
  • Organizer: 第134回日本歯科保存学会春季学術大会
  • Place of Presentation: 東京ベイ舞浜ホテルクラブリゾート(千葉)
  • Year and Date: 2011-06-10
• [Presentation] Altered protein expressions by sub-minimum inhibitory concentrations of azithromycin on Porphyromonas gingivalis biofilms
  • Author(s): Hazuki Maezono
  • Organizer: ASM 6th Conference on Biofilms
  • Place of Presentation: Miami, FL, USA
• [Presentation] Azithromycin alters protein expressions in Porphyromonas gingivalis biofilm-detached cells
  • Author(s): Hazuki Maezono
  • Organizer: 第60回 国際歯科研究学会日本部会（JADR）総会・学術大会
  • Place of Presentation: 朱鷺メッセ（新潟）