The role of adenosine to regulate osteoimmunology in inflammatory bone destruction

• Project/Area Number: 23890110
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Periodontal dentistry
• Research Institution: Osaka University
• Principal Investigator: TAKEDACHI Masahide 大阪大学, 大学院・歯学研究科, 助教 (60452447)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 歯周炎 / 骨免疫 / 炎症性骨破壊 / アデノシン / 骨代謝 / CD73 / 骨芽細胞

Research Abstract

Porphyromonas gingivalis-infected CD73 deficient mice revealed the role of CD73 in periodontitis. Osteoblasts isolated from CD73 deficient mice showed impaired differentiation compared to those from wild type mice. On the other hand, IL-1 beta decreased A2A and increased A2B adenosine receptor expression in mouse osteoblasts. Moreover, CD73 overexpression resulted in increase of RANKL expression and the expression was further enhanced when the cells were stimulated with IL-1 beta. These results suggest that endogenous adenosine is involved in inflammatory bone destruction.

Research Products

• [Journal Article] アデノシンによる骨芽細胞分化制御機構 (2012)
  • Author(s): 竹立匡秀
  • Journal Title: 大阪大学歯学雑誌 Volume: 57巻 Pages: 1-6
  • NAID: 10031118060
• [Presentation] CD73 and its role in osteoblast differentiation (2012)
  • Author(s): Masahide Takedachi, Hiroyuki Oohara, Mitsuyoshi Iyama, Linda F.Thompson, Shinya Murakami
  • Organizer: Purine 2012
  • Place of Presentation: 福岡県福岡市
  • Year and Date: 2012-05-31
• [Presentation] アデノシンによる骨芽細胞分化制御機構 (2012)
  • Author(s): 竹立匡秀
  • Organizer: 大阪大学歯学会第113回例会
  • Place of Presentation: 大阪府吹田市
  • Year and Date: 2012-01-12
• [Presentation] アデノシンによる骨芽細胞分化制御機構 (2012)
  • Author(s): 竹立匡秀
  • Organizer: 大阪大学歯学会第113回例会
  • Place of Presentation: 大阪大学歯学部(大阪府)
  • Year and Date: 2012-01-12
• [Presentation] CD73(ecto-5'-nucleotidase) に よる ア デノシン受容体活性化制御が骨芽細胞分化に及ぼす影響 (2011)
  • Author(s): 大原廣之、竹立匡秀、伊山舜吉、村上伸也
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪府大阪市
  • Year and Date: 2011-07-29
• [Presentation] CD73(ecto-5'-nucleotidase)によるアデノシン受容体活性化制御が骨芽細胞分化に及ぼす影響 (2011)
  • Author(s): 大原廣之
  • Organizer: 第29回日本骨代謝学会学術集会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2011-07-29
• [Presentation] CD73 and its role in osteoblast differentiation
  • Author(s): Masahide Takedachi, Hiroyuki Oohara, Mitsuyoshi Iyama, Linda F Thompson, Shinya Murakami
  • Organizer: Purine 2012
  • Place of Presentation: 福岡市