| Project/Area Number |
23890118
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Thoracic surgery
|
|---|
| Research Institution | Okayama University |
|---|
Principal Investigator |
UENO Tsuyoshi 岡山大学, 岡山大学病院, 医員 (80509456)
|
|---|
| Project Period (FY) |
2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
|---|
| Keywords | microRNA / microRNA-34 / 中皮腫 / 正常中皮 / mesothelioma / mesothelial cell |
|---|
| Research Abstract |
Malignant mesothelioma(MM) is an aggressive tumor with a dismal prognosis. We previously reported that microRNA-34(miR-34) are methylated and down-regulated in MM and may play an important role in the carcinogenesis of MM. In this study, we down-regulated miR-34 in human mesothelial cells to investigate the cellular effect of miR-34 knockdown. miR-34 inhibitor transfection significantly increased cell proliferation. The invasive ability also increased in the miR-34 inhibitor transfectants. the up-regulation of c-Met, phospho-c-Met, and bcl-2 proteins were shown. In conclusion, the down-regulation of miR-34 induced an oncogenic phenotype in non-malignant mesothelial cells.
|
