The role of Leukotrine B4 in dendritic cell-T cell interaction

• Project/Area Number: 23890146
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: General medical chemistry
• Research Institution: Juntendo University (2012); Kyushu University (2011)
• Principal Investigator: KOGA Tomoaki 順天堂大学, 医学部, 助教 (30615092)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: GPCR / 樹状細胞 / T 細胞 / 生理活性脂質 / BLT1 / 脂質免疫 / T細胞 / コンディショナルノックアウト / LTB4 / 免疫応答

Research Abstract

I revealed the important role of LTB4-BLT1 axis in antigen-dependent immunological synapse (IS) formation between dendritic cells (DC) and CD4+T cells. By using in vitroDC-T cell co-culture system, I also found that BLT1 in DC is important for IS formation. In parallel, to investigate the role of BLT1 in DC in vivo, I established the BLT1floxedmice. I will generate DC-specific BLT1 conditional knockout mice.

Research Products

• [Journal Article] Suppression of inflammation viaPDE4B-JNK2-dependent upregulation of deubiquitinase CYLD (2013)
  • Author(s): Komatsu K, Lee J, Miyata M, Lim JH, Jono H, Koga T, Xu H, Yan C, Kai H, Li JD
  • Journal Title: Nature Commun Volume: 4
• [Journal Article] Insulin receptor activation through its accumulation in lipid rafts by mild electrical stress (2013)
  • Author(s): Morino-Koga S, Yano S, Kondo T, Shimauchi Y, Matsuyama S, Okamoto Y, Suico MA, Koga T, Sato T, Shuto T, Arima H, Wada I, Araki E, Kai H
  • Journal Title: J Cell Physiol Volume: 228(2) Pages: 439-446
• [Journal Article] Inhibition of PDE4B suppresses inflammation by increasing expression of the deubiquitinase CYLD (2013)
  • Author(s): Komatsu K
  • Journal Title: Nature Communications Volume: 4 Issue: 1 Pages: 1684-1684
  • DOI: 10.1038/ncomms2674
  • Peer Reviewed
• [Journal Article] Mild electrical stimulation and heat shock ameliorates progressive proteinuria and renal inflammation in mouse model of Alport syndrome (2012)
  • Author(s): Koga T, Kai Y, Fukuda R, Morino-Koga S, Suico MA, Koyama K, Sato T, Shuto T, Kai H
  • Journal Title: PLoS ONE Volume: 7(8)
• [Journal Article] STT3B-dependent posttranslational N- glycosylation as a sruceillance system for secretory protein (2012)
  • Author(s): Sato T, Sako Y, Sho M, Momohara M, Suico MA, Shuto T, Nishitoh H, Okiyoneda T, Kokame K, Kaneko M, Taura M, Miyata M, Chosa K, Koga T, Morino-Koga S, Wada I, Kai H
  • Journal Title: Mol Cell Volume: 47(1) Pages: 99-110
• [Journal Article] (2012)
  • Author(s): Lim JH, Jono H, Komatsu K, Woo CH, Lee J, Miyata M, Matsuno T, Xu X, Huang Y, Zhang W, Park SH, Kim YI, Choi YD, Shen H, Heo KS, Xu H, Bourne P, Koga T, Xu H, Yan C, Wang B, Chen LF, Feng XH, Li JD.
  • Journal Title: Nature Commun
• [Journal Article] Mild electrical stimulation and heat shock ameliorates progressive proteinuria and renal inflammation in mouse model of Alport syndrome. (2012)
  • Author(s): Tomoaki Koga
  • Journal Title: PLoS One Volume: 7 Issue: 8 Pages: 43852-43852
  • DOI: 10.1371/journal.pone.0043852
  • Peer Reviewed
• [Journal Article] Insulin receptor activation through its accumulation in lipid rafts by mild electrical stress (2012)
  • Author(s): Morino-Koga S
  • Journal Title: J Cell Physiol. Volume: 228 Issue: 2 Pages: 439-446
  • DOI: 10.1002/jcp.24149
  • Peer Reviewed
• [Journal Article] STT3B-dependent posttranslational N-glycosylation as a surveillance system for secretory protein. (2012)
  • Author(s): Sato T., et.al.
  • Journal Title: Molecular Cell Volume: 47(1) Issue: 1 Pages: 99-110
  • DOI: 10.1016/j.molcel.2012.04.015
  • Peer Reviewed
• [Journal Article] CYLD Negatively Regulates Transforming Growth Factor-β Signaling via Deubiquitinating Akt (2012)
  • Author(s): Lim JH, et al
  • Journal Title: Nature Communications Volume: (in press)
  • Peer Reviewed
• [Journal Article] Lucidenic acids-rich extract from antlered form of Ganoderma lucidum enhances TNF-alpha induction in THP-1 monocytic cells possibly via its modulation of MAP kinases p38 and JNK. (2011)
  • Author(s): Watanabe K, Shuto T, Sato M, Onuki K, Mizunoe S, Suzuki S, Sato T, Koga T, Suico MA, Kai H, Ikeda T.
  • Journal Title: Biochem Biophys Res Commun Volume: 408(1) Pages: 18-24
• [Journal Article] Lucidenic acids-rich extract from antlered form of Vanoderma lucidumenhances TNFalpha induction in THP-1 monocytic cells possibly via its modulation of MAP kinases p38 and JNK (2011)
  • Author(s): Watanabe K, et al
  • Journal Title: Biochem Biophys Res Commun Volume: 408 Pages: 18-24
  • Peer Reviewed
• [Presentation] A novel dendritic cell subset that promotes Th17 differentiation. (2012)
  • Author(s): Koga T, et al.
  • Organizer: 12th Biennial International Endotoxin & Innate Immunity Society (IEIIS) Meeting
  • Place of Presentation: Tokyo
• [Presentation] BLT1 as a potential marker of mouse dendritic cell subsets. (2012)
  • Author(s): 古賀友紹
  • Organizer: 第41回日本免疫学会
  • Place of Presentation: 神戸
• [Presentation] BLT1 as a potential marker of mouse dendritic cell subsets (2011)
  • Author(s): Koga T, et al
  • Organizer: The 10^<th> JBS Biofrontier Symposium : New aspects of phospholipid biology and medicine
  • Place of Presentation: Fukuoka
• [Remarks]
  • URL: http://plaza.umin.ac.jp/j_bio/Biochem1/
• [Remarks]
  • URL: https://www.juntendo.ac.jp/graduate/kenkyudb/search/researcher.php?MID=4838
• [Remarks] 順天堂大学医学部 生化学第一講座
  • URL: http://plaza.umin.ac.jp/j_bio/Biochem1/Top.html
• [Remarks] 順天堂大学研究者情報データベース 研究者詳細
  • URL: https://www.juntendo.ac.jp/graduate/kenkyudb/search/researcher.php?MID=4838
• [Remarks]
  • URL: http://www.med.kyushu-u.ac.jp/biochem1/