Effects of IL-23 and Th17 on intestinal smooth muscle contractility and its underlying mechanism

Research Project

Project/Area Number	23890149
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	Gastroenterology
Research Institution	Kyushu University
Principal Investigator	IHARA Eikichi 九州大学, 大学病院, 助教 (80612390)
Co-Investigator(Renkei-kenkyūsha)	NAKAMURA Kazuhiko 九州大学, 大学病院, 助教 (00274449)
Project Period (FY)	2011 – 2012
Project Status	Completed (Fiscal Year 2012)
Budget Amount *help	¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000) Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords	IL-23 / IL-17 / Th17 / 消化管平滑筋 / 消化管免疫 / カルシウム感受性 / 腸管平滑筋 / K^+脱分極刺激 / MLCK / MLCP / カルバコール / alpha-toxin
Research Abstract	The objective of the present study was to investigate effects of IL-23 and IL-17, which are associated with Th17 immune response, on the intestinal smooth muscle. In the rat intestinal smooth muscle, tissue culture with IL-23 for 3 days slightly reduced contractile response to 118 mM K+, but not to carbachol. On the other hand, tissue culture with IL-17 significantly decreased the contractile responses both to 118 mM K+and carbachol. In α-toxin permeabilized smooth muscle strips, IL-17 did not change carbachol-induced Ca^2+-sensitization. These results indicated that Th17 immune response reduced contractility in the rat intestinal smooth muscle without changing Ca^2+-sensitization via a Ca^2+/calmodulin/MLCK pathway.