Development of in vivo hematopoietic differentiation system for human iPS cells

• Project/Area Number: 23890199
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Hematology
• Research Institution: Jichi Medical University
• Principal Investigator: ABE Tomoyuki 自治医科大学, 医学部, 助教 (20610364)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 再生医学 / 幹細胞 / トランスレーショナルリサーチ / 移植・再生医療 / 応用動物

Research Abstract

We have shown the safety and effectiveness of treatment with busulfan in sheep and have suggested a conditioning regimen with busulfan for sheep in utero transplantation involving intravenous administration of 3 mg/kg busulfan to pregnant ewes 6 days befo

Research Products

• [Journal Article] Generation of Naive-like Porcine Induced Pluripotent Stem Cells Capable of Contributing to Embryonic and Fetal Development (2013)
  • Author(s): Fujishiro SH, Nakano K, Mizukami Y, Azami T, Arai Y, Matsunari H, Ishino R, Nishimura T, Watanabe M, Abe T, Furukawa Y, Umeyama K, Yamanaka S, Ema M, Nagashima H, Hanazono Y
  • Journal Title: Stem Cells Dev Volume: 22(3) Issue: 3 Pages: 473-482
  • DOI: 10.1089/scd.2012.0173
  • Peer Reviewed
• [Journal Article] Maternal administration of busulfan before in utero transplantation of human hematopoietic stem cells enhances engraftments in sheep (2012)
  • Author(s): Abe T, Masuda S, Tanaka Y, Nitta S, Kitano Y, Hayashi S, Hanazono Y, Nagao Y
  • Journal Title: Exp Hematol Volume: 40(6) Issue: 6 Pages: 436-444
  • DOI: 10.1016/j.exphem.2012.01.018
  • Peer Reviewed
• [Journal Article] Migration of cells from the yolk sac to hematopoietic tissues after in utero transplantation of early and mid gestation canine fetuses (2011)
  • Author(s): Masuda S, Hayashi S, et al
  • Journal Title: Transplantation Volume: 92(2) Issue: 2 Pages: e5-e6
  • DOI: 10.1097/tp.0b013e318222119f
  • Peer Reviewed
• [Journal Article] Ex vivo expansion of human HSCs with Sendai virus vector expressing HoxB4 assessed by sheep in utero transplantation (2011)
  • Author(s): Abe T, Masuda S, et al
  • Journal Title: Exp Hematol Volume: 39(1) Issue: 1 Pages: 47-54
  • DOI: 10.1016/j.exphem.2010.09.007
  • Peer Reviewed
• [Presentation] ヒト化ヒツジの作製を目指して (2013)
  • Author(s): 阿部朋行、花園豊
  • Organizer: 第60回日本実験動物学会
  • Place of Presentation: つくば市
  • Invited
• [Presentation] ブタを用いたiPS 細胞の免疫原性に関する検証 (2012)
  • Author(s): 水上喜久, 阿部朋行, 藤城修平, 柴田宏昭, 菱川修司, 小林英司, 花園 豊
  • Organizer: 第15回日本異種移植研究会
  • Place of Presentation: 京都
  • Year and Date: 2012-12-08
• [Presentation] ヒツジにおけるヒト造血細胞の長期生着 (2012)
  • Author(s): 阿部朋行、増田茂夫、新田卓、林聡、長尾慶和、花園豊
  • Organizer: 第11回自治医科大学シンポジウム
  • Place of Presentation: 下野
  • Year and Date: 2012-08-31
• [Presentation] Generation of Naive-like Porcine Induced Pluripotent Stem Cells Capabe of Contributing to Embryonic and Fetal Development (2012)
  • Author(s): Shuh-hei Fujishiro, Kazuaki Nakano, Yoshihisa Mizukami, Takuya Azami, Yoshikazu Arai, Hitomi Matsunari, Takashi Nishimura, Masahito Watanabe, Tomoyuki Abe, Kazuhiro Umeyama, Shinya Yamanaka, Masatsugu Ema, Hiroshi Nagashima, and Yutaka Hanazono
  • Organizer: MBSJ The 35th Annual Meeting
  • Place of Presentation: Fukuoka
• [Presentation] ヒツジ胎子内におけるサルES 細胞の生着、造血系分化およびテラトーマ形成に及ぼす要因 (2012)
  • Author(s): 新田卓, 阿部朋行, 田中裕次郎, 佐々木京子, 増田茂夫, ボラジギン・サラントラガ, 林聡, 北野良博, 花園豊, 長尾慶和
  • Organizer: 第105回日本繁殖生物学会
  • Place of Presentation: 筑波
• [Presentation] Long-term Follow-up on the Engraftment of Human HSCs in Sheep after in Utero Transplantation; Society for Hematology and stem cells (2012)
  • Author(s): Yutaka Hanazono, Tomoyuki Abe, Suguru Nitta, Shigeo Masuda, Satoshi Hayashi, Yoshikazu Nagao
  • Organizer: 41st Annual Scientific Meeting
  • Place of Presentation: Amsterdam, Netherland
• [Presentation] Possible factors for engraftment of monkey embryonic stem cells after in utero transplantation into sheep fetuses (2012)
  • Author(s): Yoshikazu Nagao, Tomoyuki Abe, Yujiro Tanaka, Kyoko Sasaki, Shigeo Masuda, Suguru Nitta, Borjigin Sarentonglaga, Satoshi Hayashi, Yoshihiro Kitano, Yutaka Hanazono
  • Organizer: 17th International Congress on Animal Reproduction
  • Place of Presentation: Vancouver
• [Presentation] HoxB4-Transduction of Human HSC Results in Longer-Term Engraftment Compared to Busulfan-Conditioning in Sheep after in Utero Transplantation (2012)
  • Author(s): Tomoyuki Abe, Suguru Nitta, Shigeo Masuda, Satoshi Hayashi, Yoshikazu Nagao and Yutaka Hanazono
  • Organizer: The 18th Annual Meeting of Japan Society of Gene Therapy
  • Place of Presentation: Kumamoto, Japan
• [Presentation] Long-Term Comparative Study on the Engraftment of Human Hematopoietic Stem Cells in Sheep (2012)
  • Author(s): Tomoyuki Abe, Suguru Nitta, Shigeo Masuda, Satoshi Hayashi, Yoshikazu Nagao and Yutaka Hanazono
  • Organizer: The 10th Annual Meeting of International Society for Stem Cells Research
  • Place of Presentation: Yokohama, Japan
• [Presentation] HoxB4-Transduction of Human HSC Results in Longer-Term Engraftment Compared to Busulfan-Conditioning in Sheep after in Utero Transplantation (2012)
  • Author(s): Tomoyuki Abe, Suguru Nitta, Shigeo Masuda, Satoshi Hayashi, Yoshikazu Nagao and Yutaka Hanazono
  • Organizer: 第10回 幹細胞シンポジウム
  • Place of Presentation: 淡路
• [Presentation] ヒツジにおけるヒト造血細胞長期生着の条件検討 (2012)
  • Author(s): 阿部朋行、新田卓、増田茂夫、林聡、長尾慶和、花園豊
  • Organizer: 第59回 日本実験動物学会総会
  • Place of Presentation: 別府
• [Presentation] HoxB4-Transduction of Human HSC Results in Longer-Term Engraftment Compared to Busulfan-Conditioning in Sheep after in Utero Transplantation (2012)
  • Author(s): Tomoyuki Abe, Suguru Nitta, Shigeo Masuda, Satoshi Hayashi, Yoshikazu Nagao and Yutaka Hanazono
  • Organizer: The 18th Annual Meeting of Japan Society of Gene Therapy
  • Place of Presentation: 熊本市
• [Presentation] Long-Term Comparative Study on the Engraftment of Human Hematopoietic Stem Cells in Sheep (2012)
  • Author(s): Tomoyuki Abe, Suguru Nitta, Shigeo Masuda, Satoshi Hayashi, Yoshikazu Nagao and Yutaka Hanazono
  • Organizer: The 10th Annual Meeting of International Society for Stem Cells Research
  • Place of Presentation: 横浜市
• [Presentation] HoxB4-Transduction of Human HSC Results in Longer-Term Engraftment Compared to Busulfan-Conditioning in Sheep after in Utero Transplantation (2012)
  • Author(s): Tomoyuki Abe, Suguru Nitta, Shigeo Masuda, Satoshi Hayashi, Yoshikazu Nagao and Yutaka Hanazono
  • Organizer: 第10回 幹細胞シンポジウム
  • Place of Presentation: 兵庫
• [Presentation] ヒツジにおけるヒト造血細胞長期生着の条件検討 (2012)
  • Author(s): 阿部朋行、新田卓、増田茂夫、林聡、長尾慶和、花園豊
  • Organizer: 第59回 日本実験動物学会総会
  • Place of Presentation: 別府市
• [Presentation] ヒツジ胎子内におけるサルES細胞の生着、造血系分化およびテラトーマ形成に及ぼす要因 (2012)
  • Author(s): 新田卓, 阿部朋行, 田中裕次郎, 佐々木京子, 増田茂夫, ボラジギン・サラントラガ, 林聡, 北野良博, 花園豊, 長尾慶和
  • Organizer: 第105回日本繁殖生物学会
  • Place of Presentation: つくば市
• [Presentation] Long-term Follow-up on the Engraftment of Human HSCs in Sheep after in Utero Transplantation (2012)
  • Author(s): Yutaka Hanazono, Tomoyuki Abe, Suguru Nitta, Shigeo Masuda, Satoshi Hayashi, Yoshikazu Nagao
  • Organizer: Society for Hematology and stem cells, 41st Annual Scientific Meeting
  • Place of Presentation: Amsterdam, Netherland
• [Presentation] Possible factors for engraftment of monkey embryonic stem cells after in utero transplantation into sheep fetuses (2012)
  • Author(s): Nagao Yoshikazu, Abe Tomoyuki, Tanaka Yujiro, Sasaki Kyoko, Masuda Shigeo, Nitta Suguru, Sarentonglaga Borjigin, Hayashi Satoshi, Kitano Yoshihiro, Hanazono Yutaka
  • Organizer: 17th International Congress on Animal Reproduction
  • Place of Presentation: Vancouver, Canada
• [Presentation] Conditioning With Busulfan Confers Engraftment Potential on Human Hematopoietic Stem Cell Comparable to HoxB4 Transduction in Sheep In Utero Transplantation (2012)
  • Author(s): Tomoyuki Abe, Shigeo Masuda Nitta Suguru, Satoshi Hayashi, Yujiro Tanaka, Hiroshi Ban, Makoto Inoue, Mamoru Hasegawa, Yoshikazu Nagao, Yutaka Hanazono
  • Organizer: The 9^<th> Annual Meeting of International Society for Stem Cells Research
  • Place of Presentation: メトロトロントコンベンションセンター(カナダ、トロント)
• [Presentation] ヒツジ胎子へのブスルファン前処置によるヒト造血幹細胞移植後の造血キメラ率の向上 (2011)
  • Author(s): 阿部朋行、増田茂夫、新田卓、林聡、長尾慶和、花園豊
  • Organizer: 第14回 日本異種移植研究会
  • Place of Presentation: 広島
  • Year and Date: 2011-12-10
• [Presentation] ヒツジ胎子へのブスルファン前処置によるヒト造血幹細胞移植後の造血キメラ率の向上 (2011)
  • Author(s): 阿部朋行、増田茂夫、新田卓、林聡、長尾慶和、花園豊
  • Organizer: 第14回日本異種移植研究会
  • Place of Presentation: 広島大学応仁会館(広島県)
  • Year and Date: 2011-12-10
• [Presentation] 子宮内移植後のヒツジ体内におけるヒトおよびサル造血の長期生着に及ぼす要因 (2011)
  • Author(s): 新田卓, 阿部朋行, 増田茂夫, 林聡, 花園豊, 長尾慶和
  • Organizer: 第104回日本繁殖生物学会
  • Place of Presentation: 盛岡
• [Presentation] Conditioning With Busulfan Confers Engraftment Potential on Human Hematopoietic Stem Cell Comparable to HoxB4 Transduction in Sheep In Utero Transplantation (2011)
  • Author(s): Tomoyuki Abe, Shigeo Masuda, Nitta Suguru, Satoshi Hayashi, Yujiro Tanaka, Hiroshi Ban, Makoto Inoue, Mamoru Hasegawa, Yoshikazu Nagao, Yutaka Hanazono
  • Organizer: The 9th Annual Meeting of International Society for Stem Cells Research
  • Place of Presentation: Toronto, Ontario Canada
• [Presentation] ヒツジ胎子へのブスルファン投与はヒト造血幹細胞移植後の生着率を向上させる (2011)
  • Author(s): 阿部朋行、増田茂夫、新田卓、林聡、花園豊、長尾慶和
  • Organizer: 第58回 日本実験動物学会総会
  • Place of Presentation: 東京
• [Presentation] ヒツジ胎子へのブスルファン投与はヒト造血幹細胞移植後の生着率を向上させる (2011)
  • Author(s): 阿部朋行、増田茂夫、新田卓、林聡、花園豊、長尾慶和
  • Organizer: 第58回日本実験動物学会総会
  • Place of Presentation: タワーホール船堀(東京都)