The establishment of themethod to induce renal cellsfor renal disease specific iPS cell research

• Project/Area Number: 23890203
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: General internal medicine (including Psychosomatic medicine)
• Research Institution: Keio University
• Principal Investigator: MORIZANE Ryuji 慶應義塾大学, 医学部, 助教 (00468505)
• Project Period (FY): 2011 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 多能性幹細胞 / 胚性幹細胞 / 尿細管細胞 / 腎臓 / 人工多能性幹細胞 / 尿細管上皮細胞 / 再生医療 / KSP / 再生

Research Abstract

I have established the method to induce renal tubular cells from embryonic stem cells via purification of KSP-positive cells by use of anti-KSP antibody. Global gene expression profiles of KSP-positive cells derived from ES cells showed characteristics of

Research Products

• [Journal Article] Kidney specific protein-positive cells derived from embryonic stem cells reproduce tubular structures in vitro and differentiate into renal tubular cells (2013)
  • Author(s): Ryuji Morizane
  • Journal Title: PLOS ONE Volume: 未定
  • Peer Reviewed
• [Presentation] マウス ES 細胞を用いた尿細管細胞の分化誘導 (2012)
  • Author(s): 森實隆司、門川俊明、伊藤裕
  • Organizer: 日本腎臓学会 第55回学術総会
  • Place of Presentation: パシフィコ横浜(神奈川)
  • Year and Date: 2012-06-01
• [Presentation] マウスES細胞を用いた尿細管細胞の分化誘導 (2012)
  • Author(s): 森實 隆司
  • Organizer: 日本腎臓学会 第55回学術総会
  • Place of Presentation: 横浜
• [Presentation] KSP-Positive Cells Derived from Mouse Embryonic Stem Cells Serve as Progenitors of Renal Tubule Cells (2012)
  • Author(s): Ryuji Morizane
  • Organizer: American Society of Nephrology, Kidney Week 2012
  • Place of Presentation: San Diego
• [Presentation] Purification of Differentiated Tubule Cells from Mouse Embryonic Stem Cells Using Flow Cytometry (2011)
  • Author(s): Ryuji Morizane, Toshiaki Monkawa, Hiroshi Itoh
  • Organizer: American society of Nephrology's 44nd Annual Meeting & Scientific Exposition
  • Place of Presentation: フィラデルフィア(アメリカ
  • Year and Date: 2011-11-10
• [Presentation] Purification of Differentiated Tubule Cells from Mouse Embryonic Stem Cells Using Flow Cytometry (2011)
  • Author(s): 森實隆司
  • Organizer: American society of Nephrology's 44nd Annual Meeting & Scientific Exposition
  • Place of Presentation: アメリカ、フィラデルフィア
  • Year and Date: 2011-11-10
• [Patent(Industrial Property Rights)] 抗 KSP マウスモノクローナル抗体を用いた、胚性幹細胞から in vitro での腎構成細胞の誘導方法の開発 (2012)
  • Inventor(s): 森實隆司、門川俊明、伊藤裕
  • Industrial Property Rights Holder: 慶應義塾大学
  • Filing Date: 2012-09-25
  • Overseas
• [Patent(Industrial Property Rights)] 尿細管上皮細胞前駆細胞 (2012)
  • Inventor(s): 森實隆司、門川俊明、伊藤裕
  • Industrial Property Rights Holder: 森實隆司、門川俊明、伊藤裕
  • Industrial Property Rights Type: 特許
  • Filing Date: 2012-09-25
  • Overseas
• [Patent(Industrial Property Rights)] 抗 KSP マウスモノクローナル抗体を用いた、胚性幹細胞から in vitro での腎構成細胞の誘導方法の開発 (2011)
  • Inventor(s): 森實隆司、門川俊明、伊藤裕
  • Industrial Property Rights Holder: 慶應義塾大学
  • Filing Date: 2011-09-26
• [Patent(Industrial Property Rights)] 抗KSPマウスモノクローナル抗体を用いた、胚性幹細胞からin vitroでの腎構成細胞の誘導方法の開発 (2011)
  • Inventor(s): 森實隆司、門川俊明、伊藤裕
  • Industrial Property Rights Holder: 慶應義塾大学
  • Industrial Property Number: 2011-209792
  • Filing Date: 2011-09-26