Presynaptic cell-type-dependent regulation of GABAergic synaptic transmission by nitric oxide in rat insular cortex
| Project/Area Number |
23890216
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional basic dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 島皮質 / シナプス伝達 / 短期可塑性 / NO / 神経生理 / 味覚 / IPSC / 一酸化窒素 |
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| Research Abstract |
Nitric oxide (NO) is a key retrograde messenger that regulates synaptic transmission in the cerebral cortex. We performed multiple whole-cell patch clamp recordings from Layer V neurons in rat insular cortex. SNAP, an NO donor, suppressed uIPSC amplitudes in approximately 40% of the connections, whereas 35% of the connections showed uIPSC facilitation in inhibitory synapses composed of presynaptic fast-spiking (FS)neurons. In contrast, synaptic connections composed of presynaptic non-FS neuron types showed a constant suppression of uIPSCs by SNAP. These results suggest that NO regulation of inhibitory synaptic transmission is dependent on presynaptic cell subtypes and that its effects are partially mediated by presynaptic mechanisms.
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Report
(3 results)
Research Products
(6 results)
