| Project/Area Number |
23890230
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Thoracic surgery
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| Research Institution | Osaka Medical College |
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Principal Investigator |
KANKI Sachiko 大阪医科大学, 医学部, 助教 (40411350)
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| Project Period (FY) |
2011 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
|
| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 心筋虚血 / アフィニティ精製 / ペプチド / アフィニティー精製 / 心筋虚血再灌流傷害 / ペプチド創薬 |
|---|
| Research Abstract |
To find a receptor molecule of the ischemic heart-homing peptide, the affinity screening is necessary and the carrier must play a key role. FG beads, which are 200nm in diameter and consisted of magnetic ferrite covered by Poly-glycidylmethacrylate resin, are used as affinity carrier to bind the ischemic heart-homing peptides. The condition to bind the peptide to FG beads was optimized, and the combination of the acetylene peptide at N’-terminus and azide FG beads worked not destroy tertiary structure of peptide. Then, pull-down assay with the affinity beads was performed to find receptors for the homing peptide from ischemic heart tissue of rats, which is made by temporary occlusion of leftcoronary artery.
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