| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
We performed promoter-wide DNA methylation analysis of lymphoblastoid cell lines (LCLs) derived from two pairs of monozygotic twins discordant for bipolar disorder (BD). We found distinct difference of DNA methylation in SLC6A4 encoding serotonin transporter (HTT), which is a target of antidepressants and is one of the strongest candidate molecules of mood disorder. Promoter hypermethylation of SLC6A4 in LCLs of BD patients was confirmed in a case-control analysis. DNA methylation of SLC6A4 was significantly correlated with its mRNA expression level in individuals with the S/S genotype of serotonin transporter-linked promoter region (HTTLPR), and mRNA expression level was lower in BD patients carrying the S/S genotype. DNA methylation of the same site was also higher in the postmortem brains of BD patients.
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