| Project/Area Number |
23K05013
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 38020:Applied microbiology-related
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| Research Institution | Yokohama City University |
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Principal Investigator |
Robert Kanaly 横浜市立大学, 理学部, 教授 (00448656)
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| Co-Investigator(Kenkyū-buntansha) |
守 次朗 横浜市立大学, 理学部, 助教 (10835143)
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| Project Period (FY) |
2023-04-01 – 2026-03-31
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| Project Status |
Granted (Fiscal Year 2023)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2025: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2024: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2023: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | PAHs / asphaltenes / biotransformation / Sphingobium / Pseudomonas / 重質油改質技術 / 環境微生物 / 多環芳香族炭化水素 / アスファルテン |
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| Outline of Research at the Start |
本研究では、環境微生物に対する代謝プロファイリングとゲノム生化学解析を通して、共存する異種細菌による協調的な鎖状アルキル化多環芳香族炭化水素（CA-PAHs）生分解機構を証明すると共に、重質油改質技術への応用に向けたその実用性を評価することを目指している。具体的には、（１）異なる分子構造を持つモデルCA-PAHsに対する各細菌株の生分解能力の調査、（２）細菌株間のクロスフィーディングによる協調的CA-PAHs生分解機構の証明、（３）重質油を用いた培養試験による各細菌株の重質油改質に向けた実用性の評価 を実施する予定である。
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| Outline of Annual Research Achievements |
Determined the biotransformation capabilities of Sphingobium bacterium when it was exposed to different polyalkyl chain-alkylated monoaromatic and polycyclic aromatic hydrocarbons (PAHs) and constructed biotransformation pathways for their biodegradation by comprehensive liquid chromatography electrospray ionization tandem mass spectrometry.
Cooperative biodegradation of a model asphaltene long-chain PAH was investigated by a compartmentalized approach using bacteria belonging to the genera Pseudomonas and Sphingobium. Pseudomonas reduced the size (and molar mass) of 1-dodecylnaphthalene through subterminal alkyl chain cutting resulting in shorter alkyl chain polyaromatics that were further biotransformed by Sphingobium to ultimately produce aromatic ring-opened chain-shortened compounds.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
Determined mechanisms of biotransformation of chain-alkylated PAHs by Sphingobium. Made progress on the application of high resolution mass spectrometry (HR-MS) methods development for analyzing cooperative bacterial biotransformation of a model asphaltene compound.
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| Strategy for Future Research Activity |
Develop methods for structural predictions and docking simulations of Sphingobium and Pseudomonas oxygenase enzymes and target compounds. Conduct biodegradation experiments using heavy oil. Produce heavy oil fractions from heavy oil to be used in bacterial biotransformation assays. Develop methods for IR analyses to evaluate biotransformation of complex hydrocarbon mixtures. Investigate the biotransformation of tethered aromatic compounds.
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