Theory-driven purification of amyloid fibers by size-exclusion chromatography

Research Project

Project/Area Number	23K05712
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Review Section	Basic Section 43040:Biophysics-related
Research Institution	Kanazawa University
Principal Investigator	Hall Damien 金沢大学, ナノ生命科学研究所, 特任助教 (50447950)
Project Period (FY)	2023-04-01 – 2026-03-31
Project Status	Granted (Fiscal Year 2023)
Budget Amount *help	¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000) Fiscal Year 2025: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000) Fiscal Year 2024: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000) Fiscal Year 2023: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Keywords	amyloid / biophysical modelling / chromatography
Outline of Research at the Start	The project has three sequential phases. Phase 1 involves testing the general utility of the EPT model by low pressure SEC experiments. Phase 2 involves development and testing of an NEPT computational model of asymmetric fiber elution that is capable of both simulating and fitting SEC data. Phase 3 involves using the NEPT model to predict optimal SEC configurations which will then be tested experimentally.