Structures and dynamics of ABC transporters MsbA and LptB2FG in working with LptC during transport activities

• Project/Area Number: 23K05713
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 43040:Biophysics-related
• Research Institution: Kanazawa University
• Principal Investigator: NGO XUANKIEN 金沢大学, ナノ生命科学研究所, 特任助教 (60778190)
• Project Period (FY): 2023-04-01 – 2026-03-31
• Project Status: Granted (Fiscal Year 2023)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2025: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2024: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2023: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: ABC transporter / MsbA / LptB2FG / LptC / High-speed AFM

Outline of Research at the Start

The applicant will unveil how ATP binding and hydrolysis in the NBDs of MsbA allosterically regulate conformational transitions in the TMDs and elucidate how MsbA, LptC, and LptB2FG synergistically work together for extracting and transporting lipid A/LPS from Inner Membrane toward Outer Membrane.