Uncovering of interbacterial gene regulation via extracellular vesicles in Staphylococcus aureus and Pseudomonas aerugonosa coinfection

• Project/Area Number: 23K06525
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Review Section: Basic Section 49050:Bacteriology-related
• Research Institution: Hirosaki University
• Principal Investigator: 浅野 クリスナ 弘前大学, 医学研究科, 教授 (70598622)
• Co-Investigator(Kenkyū-buntansha): Subsomwong Phawinee 弘前大学, 医学研究科, 助教 (00883745) ; 成田 浩司 弘前大学, 医学研究科, 助教 (30419220)
• Project Period (FY): 2023-04-01 – 2026-03-31
• Project Status: Granted (Fiscal Year 2023)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2025: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2024: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2023: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: Pseudomonas aeruginosa / Extracellular vesicles / small RNAs / RNA interference / Staphylococcus aureus

Outline of Research at the Start

Staphylococcus aureus and Pseudomonas aeruginosa are major causes of polymicrobial infections that often lead to poor prognosis and difficult therapeutic management. The interactions between these bacteria have been suggested, but it is unclear how they regulate each other growth and virulence. Based on the evidence of extracellular vesicle-mediated RNA transfer, this study aims to uncover interbacterial gene regulation by RNA interference. The results will provide new insights into RNAi mechanism across bacterial species and lead to new therapeutic strategies for polymicrobial infections.