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Molecular detection of intrauterine microbial colonization in women with adenomyosis and occurrence of chronic endometritis

Research Project

Project/Area Number 23K08893
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Review Section Basic Section 56040:Obstetrics and gynecology-related
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

カーン カレク  京都府立医科大学, 医学(系)研究科(研究院), 准教授 (60336162)

Co-Investigator(Kenkyū-buntansha) 森 泰輔  京都府立医科大学, 医学(系)研究科(研究院), 教授 (00569824)
Project Period (FY) 2023-04-01 – 2026-03-31
Project Status Granted (Fiscal Year 2023)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2025: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2024: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2023: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsadenomyosis / uterine infection / inflammation / chronic endometritis / bacterial colonization / molecular method
Outline of Research at the Start

Increased intrauterine microbial colonization and occurrence of chronic endometriosis has been demonstrated previously from our laboratory. However, information on this issue in different types and subtypes of adenomyosis is still unknown. Here we plan to investigate intrauterine microbial colonization and occurrence of chronic endometriosis in women with focal and diffuse adenomyosis with and without previous treatment with GnRHa and also in women with intrinsic and extrinsic adenomyosis.

Outline of Annual Research Achievements

We evaluated microbial profiles in the endometrial samples that were collected from women with different types of adenomyosis and in women without adenomyosis. We also examined inflammatory reaction and micro-vessel density in the collected tissue samples collected from these women.
In our initial findings with 16SrDNA metagenome analysis with the endometrial samples, we found that a proportion of major microbial genera has a higher tendency in women with intrinsic adenomyosis (n=8) than that in extrinsic adenomyosis (n=6). These findings were coincided with an increased tissue infiltration of CD68-stained macrophages and CD31-stained micro-vessel density. Further analysis with increased collection of samples in each group may confirm the validity of these findings.

Current Status of Research Progress
Current Status of Research Progress

2: Research has progressed on the whole more than it was originally planned.

Reason

We are still collecting additional samples from each group of intrinsic and extrinsic adenoymosis and in control women without adenomyosis with a target of 20 samples in each group.

Strategy for Future Research Activity

1. Collection of additional samples from eutopic and ectopic endometria of women with intrinsic and extrinsic adenomyosis.
2. Perform 16S rDNA metagenome analysis to examine the pattern of different microbial profile in each group of sample.
3. Immunohistochemical analysis with antibodies against CD68, Ki-67 and CD31 in each group of tissue samples.
4. Immunohisotchemical analysis with antibody against CD138 to examines the pattern of plasma cell infiltration in each group of tissue samples.

Report

(1 results)
  • 2023 Research-status Report
  • Research Products

    (10 results)

All 2024 2023

All Journal Article (5 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (5 results) (of which Int'l Joint Research: 5 results,  Invited: 5 results)

  • [Journal Article] Bacterial infection in endometriosis: A silver-lining for the development of new non-hormonal therapy?2024

    • Author(s)
      Khan KN, de Ziegler D, Guo SW
    • Journal Title

      Hum Reprod

      Volume: 39(4) Issue: 4 Pages: 623-631

    • DOI

      10.1093/humrep/deae006

    • Related Report
      2023 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The role of innate and adaptive immunity in endometriosis2024

    • Author(s)
      Khan KN, Guo SW, Ogawa K, Fujishita A, Mori T.
    • Journal Title

      J Reprod. Immunol.

      Volume: 163 Pages: 104-116

    • DOI

      10.1016/j.jri.2024.104242

    • Related Report
      2023 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Neonatal Uterine bleeding: risk factors and it’s association with endometriosis-related symptoms later in life2024

    • Author(s)
      Ogawa K, Khan KN, Fujishita A, Kitawaki J, Mori T.
    • Journal Title

      Eur J Obstet. Gynecol. Reprod Biol.

      Volume: 293 Pages: 146-155

    • DOI

      10.1016/j.ejogrb.2023.12.033

    • Related Report
      2023 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Is neonatal uterine bleeding responsible for early-onset endometriosis?2023

    • Author(s)
      Ogawa Kanae、Khan Khaleque N、Kuroboshi Haruo、Koshiba Akemi、Shimura Koki、Tajiri Tatsuro、Fumino Shigehisa、Fujita Hiroyuki、Okubo Tomoharu、Fujiwara Yoichiro、Horiguchi Go、Teramukai Satoshi、Fujishita Akira、Itoh Kyoko、Guo Sun-Wei、Kitawaki Jo、Mori Taisuke
    • Journal Title

      Reproductive Biology and Endocrinology

      Volume: 21 Issue: 1 Pages: 56-65

    • DOI

      10.1186/s12958-023-01099-1

    • Related Report
      2023 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Association between tissue stress reaction and ACE2/TMPRSS2 expression in endometria of reproductive aged women before and during Covid-19 pandemic2023

    • Author(s)
      Ogawa K, Khan KN, Fujishita A, Kitawaki J, Mori T.
    • Journal Title

      BMC Women’s Health

      Volume: 23 Issue: 1 Pages: 229-238

    • DOI

      10.1186/s12905-023-02378-0

    • Related Report
      2023 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] A targeted educational program to improve awareness and fundamental knowledge on menstrual pain and endometriosis among young women in Asia2024

    • Author(s)
      Khan KN, Fujishita A, Mori T, Kitawaki J
    • Organizer
      The 37th Annual Meeting of Thailand Society of Obstetrics and Gynecology
    • Related Report
      2023 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Occult microscopic endometriosis: an undetectable finding in visually normal peritoneum by laparoscopy2024

    • Author(s)
      Khan KN
    • Organizer
      The 2nd International Scientific conference on Endometriosis
    • Related Report
      2023 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Patterns of menstrual cycle, menstrual pain and medication usage among young women of high-income and middle-income countries2024

    • Author(s)
      Khan KN
    • Organizer
      The 13th Annual Meeting of the Taiwan Society of Endometriosis
    • Related Report
      2023 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Pathogenesis of human adenomyosis: an update and its association with infertility2024

    • Author(s)
      Khan KN
    • Organizer
      The 12th Asian Congress on Endometriosis
    • Related Report
      2023 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Bacterial contamination hypothesis: a new concept in endometriosis2023

    • Author(s)
      Khan KN, Fujishita A, Mori T
    • Organizer
      The 11th Asian Congress on Endometriosis
    • Related Report
      2023 Research-status Report
    • Int'l Joint Research / Invited

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Published: 2023-04-13   Modified: 2024-12-25  

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