| Project/Area Number |
23K08893
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 56040:Obstetrics and gynecology-related
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
カーン カレク 京都府立医科大学, 医学(系)研究科(研究院), 准教授 (60336162)
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| Co-Investigator(Kenkyū-buntansha) |
森 泰輔 京都府立医科大学, 医学(系)研究科(研究院), 教授 (00569824)
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| Project Period (FY) |
2023-04-01 – 2026-03-31
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| Project Status |
Granted (Fiscal Year 2023)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2025: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2024: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2023: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | adenomyosis / uterine infection / inflammation / chronic endometritis / bacterial colonization / molecular method |
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| Outline of Research at the Start |
Increased intrauterine microbial colonization and occurrence of chronic endometriosis has been demonstrated previously from our laboratory. However, information on this issue in different types and subtypes of adenomyosis is still unknown. Here we plan to investigate intrauterine microbial colonization and occurrence of chronic endometriosis in women with focal and diffuse adenomyosis with and without previous treatment with GnRHa and also in women with intrinsic and extrinsic adenomyosis.
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| Outline of Annual Research Achievements |
We evaluated microbial profiles in the endometrial samples that were collected from women with different types of adenomyosis and in women without adenomyosis. We also examined inflammatory reaction and micro-vessel density in the collected tissue samples collected from these women.
In our initial findings with 16SrDNA metagenome analysis with the endometrial samples, we found that a proportion of major microbial genera has a higher tendency in women with intrinsic adenomyosis (n=8) than that in extrinsic adenomyosis (n=6). These findings were coincided with an increased tissue infiltration of CD68-stained macrophages and CD31-stained micro-vessel density. Further analysis with increased collection of samples in each group may confirm the validity of these findings.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
We are still collecting additional samples from each group of intrinsic and extrinsic adenoymosis and in control women without adenomyosis with a target of 20 samples in each group.
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| Strategy for Future Research Activity |
1. Collection of additional samples from eutopic and ectopic endometria of women with intrinsic and extrinsic adenomyosis.
2. Perform 16S rDNA metagenome analysis to examine the pattern of different microbial profile in each group of sample.
3. Immunohistochemical analysis with antibodies against CD68, Ki-67 and CD31 in each group of tissue samples.
4. Immunohisotchemical analysis with antibody against CD138 to examines the pattern of plasma cell infiltration in each group of tissue samples.
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