Deciphering the mechanism of premature aging: establishment of a co-culture model to drive cell into senescence
| Project/Area Number |
23K10910
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Review Section |
Basic Section 59040:Nutrition science and health science-related
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| Research Institution | Waseda University |
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Principal Investigator |
馬 思慧 早稲田大学, 人間総合研究センター, 次席研究員 (70974343)
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| Co-Investigator(Kenkyū-buntansha) |
賈 慧娟 東京大学, 大学院農学生命科学研究科(農学部), 特任研究員 (60456324)
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| Project Period (FY) |
2023-04-01 – 2026-03-31
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| Project Status |
Granted (Fiscal Year 2023)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2025: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2024: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2023: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | aging / crosstalk / skeletal muscle / adipose / 老化 / クロストーク |
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| Outline of Research at the Start |
Deciphering how senescent cells and tissue deliver the signals and messages, are at the nexus of mechanisms involved in longevity and age-related metabolic dysfunction. Multi-tissue/organ crosstalk, defined as the mutual biological communication between distant tissue or organs mediated by signaling factors, may transmit aging signals initiated by the early-senescent cell/tissue/organs, spreading the aging signal. The present study aims to discuss how aging signals are conveyed by senescent cells and organs, to other cells and organs, as well as the underlying mechanisms.
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| Outline of Annual Research Achievements |
This year, we successfully induced cellular senescence in muscle cells through serial passaging and detected changes in the senescence-associated secretory phenotype and related mRNA expression. The detection of senescent cells was also achieved. Additionally, we successfully isolated extracellular vesicles from the senescent cells.Our research aims to further understand the role of senescent cell-derived EVs in the aging process and their potential impact on surrounding tissues. By studying the effects of these EVs on young cells, we hope to gain insights into the mechanisms of cellular senescence and its contribution to age-related pathologies.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
Due to my transfer to a new university, there has been a slight delay in the progress of some parts of the research plan. However, in the new academic year, I will make every effort to catch up and get the project back on track as quickly as possible. I am confident that with the support of my new institution and colleagues, I will be able to overcome any temporary setbacks and continue to advance this important research.
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| Strategy for Future Research Activity |
今後の研究の推進方策 Plans for the Research Scheme:
In the new academic year, we plan to investigate the role of intercellular communication in the progression of cellular senescence by co-culturing various combinations of senescent and young cells. This will include senescent cell-young cell and young cell-senescent cell co-cultures.
To comprehensively detect gene expression changes, we will employ RNA-sequencing (RNA-seq) technology. This approach will allow us to identify key genes and pathways involved in the senescence process and the effects of intercellular communication on senescence progression.
Furthermore, we will combine these findings with genetic engineering techniques to observe the specific influence of key genes on the senescence process. By manipulating the expression of these genes, we aim to gain a deeper understanding of their roles in regulating cellular senescence and the impact of intercellular communication on this process.
Through these multifaceted approaches, we hope to unravel the complex mechanisms underlying cellular senescence and the significance of intercellular communication in the aging process. The insights gained from this research may contribute to the development of novel strategies for managing age-related diseases and promoting healthy aging.
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Report
(1 results)
Research Products
(6 results)
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[Journal Article] Effects of 4’-demethylnobiletin and 4’-demethyltangeretin on osteoclast differentiation in vitro and in a mice model of estrogen-deficient bone resorption.2023
Author(s)
Hirata M, Tominari T, Ichimaru R, Takiguchi N, Tanaka Y, Takatoya M, Arai D, Yoshinouchi S, Miyaura C, Matsumoto C, Ma S, Suzuki K, Grundler M.W. F, Inada M.
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Journal Title
Nutrients
Volume: 14
Issue: 6
Pages: 1403-1403
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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