| Project/Area Number |
23K13291
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 20020:Robotics and intelligent system-related
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| Research Institution | Nagoya University |
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Principal Investigator |
金 恩恵 名古屋大学, 未来社会創造機構, 研究員 (30972517)
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| Project Period (FY) |
2023-04-01 – 2026-03-31
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| Project Status |
Granted (Fiscal Year 2024)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2025: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2024: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2023: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | Vascular Network / Micromanipulation / 3D channel / Magntic tweezer / Vascular network / Liver tissue / Magnetic field / 3D cellular structure / 3D vascular network / tissue engineering |
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| Outline of Research at the Start |
I will construct 3D hierarchical vascular networks in centimeter-sized live tissues in vitro using magnetic fields. The target size is a liver having 13cm of diameter and hierarchical vessels from portal vein to hepatic sinusoids.
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| Outline of Annual Research Achievements |
In the second year, we further advanced our magnetic tweezer-based system to fabricate more complex and functional 3D hierarchical vascular networks in cm-scale tissue constructs. To evaluate the biological effects of vascularization, we co-cultured HUVECs with liver parenchymal cells (RLC-18 cell line) and created four types of vascular patterns: (1) no vascular network, (2) vertical channel, (3) 3D hierarchical channel, and (4) 3D hierarchical channel with active perfusion. After three weeks of culture, we quantitatively analyzed cell proliferation and viability. Although the current dataset is still limited due to the complexity of co-culture conditions, results showed that the 3D hierarchical channels, particularly with perfusion, promoted higher cell viability and better maintenance of tissue structure compared to other patterns. In addition, we refined the magnetic tweezer design to enable the construction of more diverse and stable channel architectures. Moving forward, we aim to perform additional experiments to increase data volume, detect statistically significant differences, and further validate the effectiveness of hierarchical vascularization for long-term tissue culture.
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| Current Status of Research Progress |
Current Status of Research Progress
3: Progress in research has been slightly delayed.
Reason
Due to the complexity of co-culturing two distinct cell types, fine-tuning the culture conditions has posed significant challenges, leading to a relatively limited dataset at this stage. Continuous efforts are being made to accumulate more replicates and improve experimental consistency. Further investigations are essential to achieve statistically robust results and to validate the impact of hierarchical vascularization on tissue viability and function over extended culture periods.
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| Strategy for Future Research Activity |
As the next step, we aim to fabricate a wider variety of vascular channel patterns using the improved magnetic tweezer system. In parallel, we will conduct more extensive biological experiments to increase the dataset and identify statistically significant differences across experimental conditions. This will allow us to further validate the effectiveness of hierarchical vascularization in supporting long-term cell viability and tissue functionality.
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