| Project/Area Number |
23K13651
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 28050:Nano/micro-systems-related
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| Research Institution | Waseda University |
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Principal Investigator |
MEHES GABOR 早稲田大学, 理工学術院(情報生産システム研究科・センター), 講師(任期付) (30923867)
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| Project Period (FY) |
2023-04-01 – 2026-03-31
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| Project Status |
Granted (Fiscal Year 2023)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2025: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2024: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2023: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | Microbial systems / Electron transfer / Electrode modification / Carbon nanotubes / Redox polymer / Conducting polymer / Electroactive bacteria / Bio electron transfer / Bioelectrochemistry / Functional nanomaterials / Biosensing / Transistor device |
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| Outline of Research at the Start |
Existing bioelectrical devices are not enough sensitive to detect extracellular electron transfer (細胞外電子移動; EET) from a low number of electroactive bacteria. In this project, new functionalized carbon nanotube (CNT) interfaces will be combined with organic transistor to achieve ultrasensitive detection of EET. We will functionalize CNTs; evaluate new interfaces with bacteria by electrochemical methods; fabricate organic transistor; measure EET in-vitro.
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| Outline of Annual Research Achievements |
The goal was to make electrodes that can sensitively measure the electrical signal of electroactive bacteria by maximizing the number of bacteria on the carbon electrode surface, and improve the bioelectrical interface. For this aim we utilized three types of nanomaterials: carbon nanotubes (CNTs) with polyvinyloxazoline attachment group, CNTs wrapped by charged cellulose derivatives, and redox polymers through collaborations with Kyushu University and Yamagata University. We observed a 4-fold increase in the measured current by nanomaterial interfaces compared to bare carbon felt, measured by chronoamperometry, while scanning electron microscopy showed an increased number of bacteria on the electrode. Exceeding the plan, we could extract current also from non-electroactive bacteria.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
A special point of the subproject containing CNTs was that twice we hosted a student from Yamagata University who prepared CNT-functionalized electrodes and measured the bacterial electrochemical performance with us, also teaching us a new way of chemical attachment of CNTs to our electrodes. This collaboration is still ongoing, expecting the student to visit us again to expand the scope of experiments. Currently, we are preparing three manuscripts where we will explain our new results, while performing some missing experiments to complete the scientific papers. Form the budgeting point of view, most expenses were spent on buying three potentiostat modules to speed up the lengthy electrochemical experiments.
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| Strategy for Future Research Activity |
The next stage of the project is to prepare organic electrochemical transistors (OECTs), integrate this device with the nanomaterials we researched in the first year, and then test the devices with bacteria. Luckily, we already started a new collaboration with researchers in Linkoping University, Sweden, who will supply us with the OECT devices, that we can further functionalize in our laboratory. Therefore, the project is expected to process smoothly. We will test several nanomaterials with OECTs by integrating them on the gate electrode to see which of them can deliver the best performance. The final stage, currently scheduled for the third year, will be testing our devices in artificial gut media.
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