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Unlocking the epigenetic potential of stress tolerance in rice

Research Project

Project/Area Number 23K13957
Research Category

Grant-in-Aid for Early-Career Scientists

Allocation TypeMulti-year Fund
Review Section Basic Section 39040:Plant protection science-related
Research InstitutionOkinawa Institute of Science and Technology Graduate University

Principal Investigator

FURCI Leonardo  沖縄科学技術大学院大学, 植物エピジェネティクスユニット, ポストドクトラルスカラー (00973471)

Project Period (FY) 2023-04-01 – 2024-03-31
Project Status Discontinued (Fiscal Year 2023)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2024: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2023: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywordsepigenetics / rice / pathogen resistance / crop protection / DNA methylation / N-6 adenine methylation
Outline of Research at the Start

Arabidopsis research has shown hypomethylated epialleles are sufficient to cause resistance to biotic stresses without genetic modifications. The project will use of a novel molecular tool to induce adjustable levels of DNA hypomethylation in rice, without lethality or sterility. Rice plants with stable epialleles will be screened for resistance against pathogens. The project will optimize a novel breeding tool for epigenetically engineering of rice. The resulting resistant lines can be directly exploited to mine for epigenetically controlled disease resistance or other attractive crop traits.

Outline of Annual Research Achievements

the grant got shortened by change to JSPS international fellowship on the same project. still multiple achievements were obtained, crucial to progress the reasearch further. A: obtained transgenic rice plants with estradiol-inducible DNA demethylation vector (Inplanta Innovations inc). The plants are now being treated with estradiol,propagated to be tested for stable changes in DNA methylation and resistance to (a)biotic stresses.B: obtained reagents for RNA and DNA methylation sequencing (NEB). soon these will be used to identify key epialleles in reistant plants. C: used Oxford Nanopore R9 cells to sequence RNA to identify for the first time high.resolution changes in N6-adenine methylation during pathogen stress, and its role in resistance. the work has been submitted to Nature Plants

Report

(1 results)
  • 2023 Annual Research Report
  • Research Products

    (2 results)

All 2024 2023

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (1 results)

  • [Journal Article] RNA N6-adenine methylation dynamics impact Hyaloperonospora arabidopsidis resistance in Arabidopsis2024

    • Author(s)
      Furci, Berthelier and Saze
    • Journal Title

      Molecular Plant

      Volume: na

    • Related Report
      2023 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] RNA N6-adenine methylation dynamics modulate Arabidopsis resistance against H. arabidopsidis2023

    • Author(s)
      Furci Leonardo
    • Organizer
      学術変革領域研究(A)「不均一環境と植物」 第 3 回若手の会 函館
    • Related Report
      2023 Annual Research Report

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Published: 2023-04-13   Modified: 2024-12-25  

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