Project/Area Number |
23K13957
|
Research Category |
Grant-in-Aid for Early-Career Scientists
|
Allocation Type | Multi-year Fund |
Review Section |
Basic Section 39040:Plant protection science-related
|
Research Institution | Okinawa Institute of Science and Technology Graduate University |
Principal Investigator |
FURCI Leonardo 沖縄科学技術大学院大学, 植物エピジェネティクスユニット, ポストドクトラルスカラー (00973471)
|
Project Period (FY) |
2023-04-01 – 2024-03-31
|
Project Status |
Discontinued (Fiscal Year 2023)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2024: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2023: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
Keywords | epigenetics / rice / pathogen resistance / crop protection / DNA methylation / N-6 adenine methylation |
Outline of Research at the Start |
Arabidopsis research has shown hypomethylated epialleles are sufficient to cause resistance to biotic stresses without genetic modifications. The project will use of a novel molecular tool to induce adjustable levels of DNA hypomethylation in rice, without lethality or sterility. Rice plants with stable epialleles will be screened for resistance against pathogens. The project will optimize a novel breeding tool for epigenetically engineering of rice. The resulting resistant lines can be directly exploited to mine for epigenetically controlled disease resistance or other attractive crop traits.
|
Outline of Annual Research Achievements |
the grant got shortened by change to JSPS international fellowship on the same project. still multiple achievements were obtained, crucial to progress the reasearch further. A: obtained transgenic rice plants with estradiol-inducible DNA demethylation vector (Inplanta Innovations inc). The plants are now being treated with estradiol,propagated to be tested for stable changes in DNA methylation and resistance to (a)biotic stresses.B: obtained reagents for RNA and DNA methylation sequencing (NEB). soon these will be used to identify key epialleles in reistant plants. C: used Oxford Nanopore R9 cells to sequence RNA to identify for the first time high.resolution changes in N6-adenine methylation during pathogen stress, and its role in resistance. the work has been submitted to Nature Plants
|